Tools
ToolsComerlato, Carolina Baldisserotto, Zhang, Xu, Walker, Krystal, Brandelli, Adriano and Figeys, Daniel (2020) Comparative proteomic analysis reveals metabolic variability of probiotic Enterococcus durans during aerobic and anaerobic cultivation. Journal of Proteomics, 220. pp. 1-8. ISSN 1874-3919
Full text not available from this repository. (Request a copy)Abstract
The variation in the bioavailability of oxygen constitutes the environmental conditions found by bacteria in their passage through the host gastro-intestinal tract. Given the importance of oxygen in the defense mechanism of bacteria, it is important to understand how bacteria respond to this stress at a metabolic level. The probiotic strain Enterococcus durans LAB18S was cultivated under aerobic and anaerobic conditions using prebiotic oligosaccharides as carbon source. The whole cell proteome and secretome were analyzed through label-free quantitative proteomics approach. The results showed that the LAB18S isolate when grown with fructo-oligosacchrides (FOS) showed a higher number of differentially expressed proteins compared to samples with galacto-oligosaccharides (GOS) or glucose. Clinically important enzymes for the treatment of cancer, L-asparaginase and arginine deiminase, were overexpressed when the isolate was cultured in FOS. In addition, the absence of oxygen induced the strain to produce proteins related to cell multiplication, cell wall integrity and resistance, and H2O2 detoxification. This study showed that E. durans LAB18S growing on FOS was stimulated to produce clinically important biomolecules, including proteins that have been investigated as potential antineoplastic agents. Significance: The probiotic strain E. durans LAB18S produce clinically relevant enzymes for the treatment of cancer when cultivated in symbiosis with fructo-oligosacchrides (FOS). In addition, proteins associated with cellular multiplication, cell wall integrity and resistance, and H2O2 detoxification were induced under anaerobic growth. These characteristics could be relevant to support maintenance of intestinal health.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | enterococcus,prebiotics,probiotics,proteomics,biophysics,biochemistry ,/dk/atira/pure/subjectarea/asjc/1300/1304 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 16 Mar 2026 16:30 |
| Last Modified: | 16 Mar 2026 16:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/102356 |
| DOI: | 10.1016/j.jprot.2020.103764 |
Actions (login required)
 |
View Item |