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ToolsWang, Jiaqin, Zhang, Xu, Li, Leyuan, Ning, Zhibin, Mayne, Janice, Schmitt-Ulms, Cian, Walker, Krystal, Cheng, Kai and Figeys, Daniel (2020) Differential Lysis Approach Enables Selective Extraction of Taxon-Specific Proteins for Gut Metaproteomics. Analytical Chemistry, 92 (7). pp. 5379-5386. ISSN 0003-2700
Full text not available from this repository. (Request a copy)Abstract
Changes in microbiome composition and function have been linked to human health and diseases. Metaproteomics provides invaluable functional information on the state of a microbiome. However, lower-abundance bacteria in complex microbiomes are difficult to observe by metaproteomics. In this study, stepwise differential lysis protocols were developed for human stool microbiomes to separate different microbial species and to increase the depth of metaproteomic measurements. We achieved differential lysis of Gram-positive (G+) and Gram-negative (G-) bacteria, selective enrichment of specific bacteria, and functional enrichment by our stepwise differential lysis protocols. Therefore, differential lysis can serve as a fractionation method to reduce sample complexity and selectively extract proteins from specific taxa for deep metaproteomic studies.
| Item Type: | Article |
|---|---|
| Additional Information: | The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.analchem.0c00062. |
| Uncontrolled Keywords: | analytical chemistry ,/dk/atira/pure/subjectarea/asjc/1600/1602 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 16 Mar 2026 13:30 |
| Last Modified: | 16 Mar 2026 13:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/102347 |
| DOI: | 10.1021/acs.analchem.0c00062 |
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