Evaluating live microbiota biobanking using an ex vivo microbiome assay and metaproteomics

Zhang, Xu, Walker, Krystal, Mayne, Janice, Li, Leyuan, Ning, Zhibin, Stintzi, Alain and Figeys, Daniel ORCID: https://orcid.org/0000-0002-5373-7546 (2022) Evaluating live microbiota biobanking using an ex vivo microbiome assay and metaproteomics. Gut Microbes, 14 (1). pp. 1-13. ISSN 1949-0976

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Abstract

Biobanking of live microbiota is becoming indispensable for mechanistic and clinical investigations of drug–microbiome interactions and fecal microbiota transplantation. However, there is a lack of methods to rapidly and systematically evaluate whether the biobanked microbiota maintains their cultivability and functional activity. In this study, we use a rapid ex vivo microbiome assay and metaproteomics to evaluate the cultivability and the functional responses of biobanked microbiota to treatment with a prebiotic (fructo-oligosaccharide, FOS). Our results indicate that the microbiota cultivability and their functional responses to FOS treatment were well maintained by freezing in a deoxygenated glycerol buffer at −80°C for 12 months. We also demonstrate that the fecal microbiota is functionally stable for 48 hours on ice in a deoxygenated glycerol buffer, allowing off-site fecal sample collection and shipping to laboratory for live microbiota biobanking. This study provides a method for rapid evaluation of the cultivability of biobanked live microbiota. Our results show minimal detrimental influences of long-term freezing in deoxygenated glycerol buffer on the cultivability of fecal microbiota.

Item Type:	Article
Additional Information:	All MS proteomics data along with the MetaLab search results were deposited to the ProteomeXchange Consortium (http://www.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PXD028857.
Uncontrolled Keywords:	biobanking,freezing,gut microbiota,metaproteomics,microbiome assay,microbiology,microbiology (medical),gastroenterology,infectious diseases,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Related URLs:	https://www.scopus.com/pages/publication...
Depositing User:	LivePure Connector
Date Deposited:	06 Mar 2026 14:30
Last Modified:	18 Jun 2026 20:55
URI:	https://ueaeprints.uea.ac.uk/id/eprint/102241
DOI:	10.1080/19490976.2022.2035658

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