Evaluating live microbiota biobanking using an ex vivo microbiome assay and metaproteomics

Zhang, Xu, Walker, Krystal, Mayne, Janice, Li, Leyuan, Ning, Zhibin, Stintzi, Alain and Figeys, Daniel (2022) Evaluating live microbiota biobanking using an ex vivo microbiome assay and metaproteomics. Gut Microbes, 14 (1). pp. 1-13. ISSN 1949-0976

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Abstract

Biobanking of live microbiota is becoming indispensable for mechanistic and clinical investigations of drug–microbiome interactions and fecal microbiota transplantation. However, there is a lack of methods to rapidly and systematically evaluate whether the biobanked microbiota maintains their cultivability and functional activity. In this study, we use a rapid ex vivo microbiome assay and metaproteomics to evaluate the cultivability and the functional responses of biobanked microbiota to treatment with a prebiotic (fructo-oligosaccharide, FOS). Our results indicate that the microbiota cultivability and their functional responses to FOS treatment were well maintained by freezing in a deoxygenated glycerol buffer at −80°C for 12 months. We also demonstrate that the fecal microbiota is functionally stable for 48 hours on ice in a deoxygenated glycerol buffer, allowing off-site fecal sample collection and shipping to laboratory for live microbiota biobanking. This study provides a method for rapid evaluation of the cultivability of biobanked live microbiota. Our results show minimal detrimental influences of long-term freezing in deoxygenated glycerol buffer on the cultivability of fecal microbiota.

Item Type:	Article
Additional Information:	All MS proteomics data along with the MetaLab search results were deposited to the ProteomeXchange Consortium (http://www.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PXD028857.
Uncontrolled Keywords:	biobanking,freezing,gut microbiota,metaproteomics,microbiome assay,microbiology,microbiology (medical),gastroenterology,infectious diseases,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	06 Mar 2026 14:30
Last Modified:	09 Mar 2026 16:31
URI:	https://ueaeprints.uea.ac.uk/id/eprint/102241
DOI:	10.1080/19490976.2022.2035658

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