Wnt5a inhibited human trophoblast cell line HTR8/SVneo invasion: implications for early placentation and preeclampsia

Chen, Ying, Zhang, Yi, Deng, Qinyin, Shan, Nan, Peng, Wei, Luo, Xin, Zhang, Hua, Baker, Philip N., Tong, Chao and Qi, Hongbo (2016) Wnt5a inhibited human trophoblast cell line HTR8/SVneo invasion: implications for early placentation and preeclampsia. Journal of Maternal-Fetal and Neonatal Medicine, 29 (21). pp. 3532-3538. ISSN 1476-7058

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Abstract

Objective: Wnt5a and Wnt signaling play potential roles in human placental and fetal development. The objective of this study is to explore the role of Wnt5a in the invasion of the human trophoblast cell line HTR8/SVneo and the probable mechanism of early placentation and preeclampsia in which Wnt5a is involved.  Methods: Human first trimester villous tissues from normal pregnancies and third trimester placentas from pregnancies with or without preeclampsia (PE) were used in the detection of the expression and subcellular location of Wnt5a. The human trophoblast cell line HTR8/SVneo was treated with 0–400 ng/ml recombinant Wnt5a to investigate the role of Wnt5a in human trophoblast invasion.  Results: Human first trimester villous is accompanied by the decreased expression of Wnt5a compared with term placenta. Upregulated Wnt5a was detected in PE placenta compared with the normal control. Wnt5a inhibited the migration and invasion of HTR8/SVneo cells with decreased integrin β1, α5 and N-cadherin. Moreover, Wnt5a downregulated β-catenin in HTR8/SVneo cells.  Conclusions: These findings strongly suggest that Wnt5a inhibits the invasion of HTR8/SVneo cells. Decreased Wnt5a facilitates early placentation, whereas increased Wnt5a contributes to the pathogenesis of PE with insufficient trophoblast invasion. Aberrant Wnt5a may function by impairing Wnt non-canonical/β-catenin signaling pathway in trophoblasts.

Item Type:	Article
Additional Information:	Publisher Copyright: © 2016 Informa UK Limited, trading as Taylor & Francis Group.
Uncontrolled Keywords:	invasion,preeclampsia,trophoblast,wnt5a,pediatrics, perinatology, and child health,obstetrics and gynaecology ,/dk/atira/pure/subjectarea/asjc/2700/2735
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	02 Mar 2026 15:30
Last Modified:	02 Mar 2026 15:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/102132
DOI:	10.3109/14767058.2016.1138102

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