Effect of chronic arsenic exposure on mouse brain tissue and serum metabolomics

Dai, Hua, Xia, Yin Yin, Ting-Li Han, Ting Li Han, Philip N Baker, Philip N.Baker, Tang, Xu, Zhang, Rui Yuan, Du, Hang, Cai, Tong Jian and Cheng, Shu Qun (2016) Effect of chronic arsenic exposure on mouse brain tissue and serum metabolomics. Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 36 (9). pp. 1192-1197. ISSN 1673-4254

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Abstract

METHODS: Twelve 3-week-old male C57BL/6J mice were randomly assigned into exposure group and control group and exposed to sodium arsenite (50 mg/L) via drinking water and deionized water for 12 weeks, respectively. After the exposure, arsenic level in the cerebrum was determined by hydride generation-atomic fluorescence spectrometry. The metabolites in the cerebral cortex and serum were determined using gas chromatography-mass spectrometry (GC/MS) analysis. Principal component analysis (PCA) was used to analyze the difference of the metabolites between the exposure and the control groups. Online tools for analyzing metabolic pathways were used to identify the related metabolites pathways. RESULTS: Arsenic content in the brain of exposure group was significantly higher than that in the control group (P<0.05). The mice exposed to arsenic had a higher level of citric acid, phenylalanine, tyrosine, histidine and lysine in the cerebral cortex (P<0.05). Serum levels of serine, glycine, proline, aspartate and glutamate were significantly higher while α-ketoglutaric acid level was significantly lower in the exposure group than in the control group (P<0.05). PCA analysis showed a significant difference in cerebral cortex and serum metabolites between the two groups. CONCLUSION: Chronic arsenic exposure may affect the function of the central nervous system by interfering with amino acid metabolism and tricarboxylic acid cycle, which may be one of the mechanisms of arsenic neurotoxicity. OBJECTIVE: To observe the effect of chronic arsenic exposure on cerebral cortex and serum metabolics of mice and explore the mechanism of arsenic neurotoxicity.

Item Type:	Article
Uncontrolled Keywords:	medicine(all) ,/dk/atira/pure/subjectarea/asjc/2700
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	02 Mar 2026 12:30
Last Modified:	02 Mar 2026 12:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/102124
DOI:	issn:1673-4254

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