Association of a reduction of G-protein coupled receptor 30 expression and the pathogenesis of preeclampsia

Feng, Xiang, Zhou, Liyuan, Mao, Xun, Tong, Chao, Chen, Xuyang, Zhao, Diqi, Baker, Philip N., Xia, Yinyin and Zhang, Hua (2017) Association of a reduction of G-protein coupled receptor 30 expression and the pathogenesis of preeclampsia. Molecular Medicine Reports, 16 (5). pp. 5997-6003. ISSN 1791-2997

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Abstract

Preeclampsia is a pregnancy-specific disorder, which is a leading cause of maternal and perinatal mortality and morbidity. A lower increase of estrogen, compared with the increase in progesterone, is associated with pathogenesis of the disease during pregnancy. G-protein-coupled receptor 30 (GPR30) mediates the action of estrogen, however remains to be investigated in preeclampsia. The levels of GPR30 were measured in placentae from uncomplicated pregnancies and pregnancies complicated by preeclampsia using immunohistochemistry and western blotting. GPR30 expression was additionally measured in placental HTR8/SVneo cells following 17β-estrogen (E2) treatment in normal or hypoxia-reoxygenation conditions by western blotting. In addition, the outgrowth of HTR8/SVneo cells following E2 treatment in hypoxia-reoxygenation conditions was measured. Levels of GPR30 were significantly reduced in placentae from women with preeclampsia as compared with uncomplicated pregnancies. Treatment with E2 significantly increased the expression of GPR30 in HTR8/SVneo cells, in normal and hypoxia-reoxygenation conditions. Furthermore, treatment with E2 increased the outgrowth of HTR8/SVneo cells in hypoxia-reoxygenation conditions. The present study demonstrated lowered placental expression of GPR30 in preeclampsia. Estrogen treatment increases GPR30 expression in extravillous trophoblast and GPR30 may be involved in extravillous trophoblast invasion.

Item Type:	Article
Additional Information:	The authors would like to thank Dr. Joanna Stanley, from The University of Auckland (Auckland, New Zealand) for editing this manuscript.
Uncontrolled Keywords:	estrogen,extravillous trophoblast,gpr30,placenta,preeclampsia,biochemistry,molecular medicine,molecular biology,genetics,oncology,cancer research,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/1300/1303
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	19 Feb 2026 11:30
Last Modified:	19 Feb 2026 11:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/101983
DOI:	10.3892/mmr.2017.7341

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