Integrated epigenetics, transcriptomics, and metabolomics to analyze the mechanisms of benzo[a]pyrene neurotoxicity in the hippocampus

Wang, Jing, Li, Chun Lin, Tu, Bai Jie, Yang, Kai, Mo, Ting Ting, Zhang, Rui Yuan, Cheng, Shu Qun, Chen, Cheng Zhi, Jiang, Xue Jun, Han, Ting Li, Peng, Bin, Baker, Philip N. and Xia, Yin Yin (2018) Integrated epigenetics, transcriptomics, and metabolomics to analyze the mechanisms of benzo[a]pyrene neurotoxicity in the hippocampus. Toxicological Sciences, 166 (1). pp. 65-81. ISSN 1096-6080

Full text not available from this repository. (Request a copy)

Abstract

Benzo[a]pyrene (B[a]P) is a common environmental pollutant that is neurotoxic to mammals, which can cause changes to hippocampal function and result in cognitive disorders. The mechanisms of B[a]P-induced impairments are complex .To date there have been no studies on the association of epigenetic, transcriptomic, and metabolomic changes with neurotoxicity after B[a]P exposure. In the present study, we investigated the global effect of B[a]P on DNA methylation patterns, noncoding RNAs (ncRNAs) expression, coding RNAs expression, and metabolites in the rat hippocampus. Male Sprague Dawley rats (SD rats) received daily gavage of B[a]P (2.0 mg/kg body weight [BW]) or corn oil for 7 weeks. Learning and memory ability was analyzed using the Morris water maze (MWM) test and change to cellular ultrastructure in the hippocampus was analyzed using electron microscope observation. Integrated analysis of epigenetics, transcriptomics, and metabolomics was conducted to investigate the effect of B[a]P exposure on the signaling and metabolic pathways. Our results suggest that B[a]P could lead to learning and memory deficits, likely as a result of epigenetic and transcriptomic changes that further affected the expression of CACNA1C, Tpo, etc. The changes in expression ultimately affecting LTP, tyrosine metabolism, and other important metabolic pathways.

Item Type:	Article
Uncontrolled Keywords:	benzo[a]pyrene,epigenetics,hippocampus,metabolomics,neurotoxicity,transcriptomics,toxicology ,/dk/atira/pure/subjectarea/asjc/3000/3005
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	27 Jan 2026 11:30
Last Modified:	27 Jan 2026 11:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/101738
DOI:	10.1093/toxsci/kfy192

Actions (login required)

View Item