Down-regulation of laminin (LN)- α5 is associated with preeclampsia and impairs trophoblast cell viability and invasiveness through PI3K signaling pathway

Zhang, Xue Mei, Xiong, Xi, Tong, Chao, Li, Qin, Huang, Shuai, Li, Qing Shu, Liu, Ya Ming, Li, Hai Ying, Baker, Phillip, Shan, Nan and Qi, Hong Bo (2018) Down-regulation of laminin (LN)- α5 is associated with preeclampsia and impairs trophoblast cell viability and invasiveness through PI3K signaling pathway. Cellular Physiology and Biochemistry, 51 (5). pp. 2030-2040. ISSN 1015-8987

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Abstract

Background/Aims: Preeclampsia (PE) is a gestational disorder defined as hypertension and proteinuria, which is deemed a major cause of maternal and neonatal mortality and morbidity worldwide. The aim of this study was to investigate the expression patterns of placental laminin (LN)-α5 expression in normal and PE pregnancies, as well as evaluating the effects of LN-α5 on trophoblast proliferation, apoptosis, and invasion. Methods: LN-α5 expression levels were examined by reverse-transcriptase polymerase chain reaction (RT-PCR), and further confirmed by western blotting and immunofluorescence staining. Cell proliferation and apoptosis were measured by CCK-8 assay and flow cytometry. Cell invasion was assessed by matrigel-based transwell assay. LN-α5 DNA methylation in placentas was determined by bisulfite sequencing PCR (BSP).  Results: LN-α5 expression levels in PE placentas were significantly lower than that of normal pregnancies. Deficiency in LN-α5 expression resulted in decreased trophoblast proliferation and invasion but increased cell apoptosis, meanwhile, PI3K/AKT/mTOR signaling pathway was impaired by LN-α5 silencing. LN-α5 promoter methylation didn't show significant difference between PE and normal placentas.  Conclusion: LN-α5 downregulation is associated with PE placenta and impairs trophoblast viability and invasiveness, which could be a causative factor of PE pathogenesis.

Item Type:	Article
Uncontrolled Keywords:	laminin (ln)- α5,pi3k signaling pathway,preeclampsia,trophoblast,medicine(all),sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2700
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	22 Jan 2026 17:30
Last Modified:	27 Jan 2026 13:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/101686
DOI:	10.1159/000495822

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