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ToolsForeman, Megan A., Smith, Janet and Publicover, Stephen J. (2006) Characterisation of serum-induced intracellular Ca2+ oscillations in primary bone marrow stromal cells. Journal of Cellular Physiology, 206 (3). pp. 664-671. ISSN 0021-9541
Full text not available from this repository. (Request a copy)Abstract
Intracellular Ca2+ signalling is pivotal to cell function and [Ca2+]i oscillations permit precise and prolonged modulation of an array of Ca2+-sensitive processes without the need for extended, global elevations in [Ca2+]i. We have studied [Ca2+]i signalling in primary rat marrow stromal cells exposed to foetal calf serum (FCS) constituents at concentrations up to those required to promote growth and differentiation in culture. Spontaneous [Ca2+]i signalling was not observed, but exposure to 1% FCS induced regular, sustained Ca2+ oscillations in 41 ± 3% of cells. Incidence of FCS-induced oscillations was dose-dependent, saturating at 0.5%. These oscillations were arrested by disruption of Ca2+ stores with 100 nM-1 μM thapsigargin or discharge of mitochondrial membrane potential and were sensitive to blockade of IP3-receptors by 50 μM 2-amino-ethoxydiphenyl borate (2-APB) and inhibition of phospholipase C with 5 μM U73122. The oscillations decreased in frequency and amplitude following inhibition of Ca2+ influx with EGTA or La3+ but were poorly sensitive to nifedipine (1-10 μM) and Bay K 8644 (300 nM). The factor(s) responsible for inducing [Ca2+]i oscillations are heat stable, insensitive to disulphide bond reduction with 20 mM dithioerythritol and retained by a 30 kDa molecular weight filter. Serum is routinely present in culture medium at 10%-15% [v/v] and marrow stromal cells maintained under culture conditions exhibited sustained oscillations. This is the first demonstration of agonist-induced complex Ca2+ signals in marrow stromal cells. We conclude that Ca2+ oscillations occur constantly in these cells in culture and are potentially important regulators of cell proliferation and differentiation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | physiology,clinical biochemistry,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1314 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 18 Dec 2025 10:30 |
| Last Modified: | 19 Dec 2025 10:33 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/101461 |
| DOI: | 10.1002/jcp.20521 |
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