Preliminary assessment of whole-blood, red-cell and platelet- leucodepleting filters for possible induction of prion release by leucocyte fragmentation during room temperature processing

Prowse, Chris V., Hornsey, V. S., Drummond, O., MacGregor, I. R., Pepper, D. S., Barclay, G. R., Bethel, H., Walker, B., Barnard, G., Kirby, L. and Hope, J. (1999) Preliminary assessment of whole-blood, red-cell and platelet- leucodepleting filters for possible induction of prion release by leucocyte fragmentation during room temperature processing. British Journal of Haematology, 106 (1). pp. 240-247. ISSN 0007-1048

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Abstract

Universal leucodepletion is being introduced in the U.K. to reduce a theoretical risk of Creutzfeldt-Jakob disease (CJD) transmission. If CJD infectivity is associated with leucocytes, any cell fragmentation associated with filtration could reduce the potential benefit. Four types each of whole blood, red cell and platelet leucodepletion filters were assessed after holding of blood units for at least 4 h at 22°C. In all cases the mean residual leucocyte content was <1 000 000 per unit, with only two individual filtered whole blood units having a leucocyte content exceeding this. Evidence of leucocyte fragmentation during filtration was sought but not found by assay of soluble elastase, beta-thromboglobulin and normal prion protein, as well as by isotopic labelling of leucocyte external membrane. These preliminary studies indicate that it was possible to prepare leucodepleted blood components by filtration at room temperature, and that this appeared not to be associated with overt cell fragmentation. Definitive demonstration that fragmentation does not occur requires the development of improved general (non-specific) assays for cell membrane fragments.

Item Type:	Article
Uncontrolled Keywords:	blood,cjd,filter,leucocyte,prion,hematology ,/dk/atira/pure/subjectarea/asjc/2700/2720
Faculty \ School:	Faculty of Science > School of Biological Sciences
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	08 Dec 2025 15:32
Last Modified:	14 Dec 2025 07:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/101312
DOI:	10.1046/j.1365-2141.1999.01530.x

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