Application of a time-resolved fluoroimmunoassay for the analysis of normal prion protein in human blood and its components

MacGregor, Ian, Hope, James ORCID: https://orcid.org/0000-0003-2015-2389, Barnard, Geoff, Kirby, Louise, Drummond, Olive, Pepper, Duncan, Hornsey, Valerie, Barclay, Robin, Bessos, Hagop, Turner, Marc and Prowse, Christopher (1999) Application of a time-resolved fluoroimmunoassay for the analysis of normal prion protein in human blood and its components. Vox Sanguinis, 77 (2). pp. 88-96. ISSN 0042-9007

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Abstract

Background and Objectives: To quantify the cellular isoform of prion protein (PrP(c)) in human blood using a new time-resolved dissociation-enhanced fluoroimmunoassay (DELFIA®). Materials and Methods: The DELFIA was optimised for human blood samples and applied to isolated cell and plasma fractions from blood donations. The physicochemical properties of PrP(c) were analysed. Results: 26.5% of blood PrP(c) was associated with the platelet fraction, 0.8% with polymorphonuclear leucocytes, 2.4% with mononuclear leucocytes, 1.8% with red cells and 68.5% with plasma (mean values from 4 processed donations). Conclusion: The majority of blood PrP(c) is found in the platelet and plasma compartments.

Item Type:	Article
Uncontrolled Keywords:	hematology ,/dk/atira/pure/subjectarea/asjc/2700/2720
Faculty \ School:	Faculty of Science > School of Biological Sciences
Related URLs:	https://www.scopus.com/pages/publication...
Depositing User:	LivePure Connector
Date Deposited:	05 Dec 2025 13:30
Last Modified:	18 Jun 2026 20:48
URI:	https://ueaeprints.uea.ac.uk/id/eprint/101289
DOI:	10.1159/000031082

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