Application of a time-resolved fluoroimmunoassay for the analysis of normal prion protein in human blood and its components

MacGregor, Ian, Hope, James, Barnard, Geoff, Kirby, Louise, Drummond, Olive, Pepper, Duncan, Hornsey, Valerie, Barclay, Robin, Bessos, Hagop, Turner, Marc and Prowse, Christopher (1999) Application of a time-resolved fluoroimmunoassay for the analysis of normal prion protein in human blood and its components. Vox Sanguinis, 77 (2). pp. 88-96. ISSN 0042-9007

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Abstract

Background and Objectives: To quantify the cellular isoform of prion protein (PrP(c)) in human blood using a new time-resolved dissociation-enhanced fluoroimmunoassay (DELFIA®). Materials and Methods: The DELFIA was optimised for human blood samples and applied to isolated cell and plasma fractions from blood donations. The physicochemical properties of PrP(c) were analysed. Results: 26.5% of blood PrP(c) was associated with the platelet fraction, 0.8% with polymorphonuclear leucocytes, 2.4% with mononuclear leucocytes, 1.8% with red cells and 68.5% with plasma (mean values from 4 processed donations). Conclusion: The majority of blood PrP(c) is found in the platelet and plasma compartments.

Item Type:	Article
Uncontrolled Keywords:	hematology ,/dk/atira/pure/subjectarea/asjc/2700/2720
Faculty \ School:	Faculty of Science > School of Biological Sciences
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	05 Dec 2025 13:30
Last Modified:	05 Dec 2025 13:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/101289
DOI:	10.1159/000031082

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