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Suh, Tae Young, Roh, In Soon, Kim, Hyo Jin, Griffiths, Peter C., Park, Kyung Je, Park, Hoo Chang, Hope, James 
ORCID: https://orcid.org/0000-0003-2015-2389, Kang, Hae Eun, Kim, Dae Yong and Sohn, Hyun Joo
(2017)
Biological and biochemical characterization of M2B cells:Classical BSE prion is conserved in transgenic mice overexpressing bovine prion protein gene.
Prion, 11 (6).
pp. 405-414.
ISSN 1933-6896
Abstract
M2B cells with persistent classical bovine spongiform encephalopathy (C-BSE) have been established previously. In this study, we performed strain characterization of the M2B cell line in bovine PrPC overexpressing mice (Tg 1896). Mice intracranially inoculated with M2B cells and C-BSE survived for 451 ± 7 and 465 ± 31 d post inoculation, respectively. Although biochemical properties, including deglycosylation and conformational stability, differed between M2B cells and C-BSE, inoculation with M2B cell lysate and C-BSE resulted in comparable phenotypes. Comparable vacuolation scores and PrPSc depositions were observed in the brain of Tg 1896 inoculated with both M2B cell lysate and C-BSE. Our results show that biochemical and biological characteristics of M2B cells and C-BSE are classifiable in the same strain.
| Item Type: | Article |
|---|---|
| Additional Information: | Publisher Copyright: © 2017 Crown copyright and Animal and Plant Quarantine Agency. |
| Uncontrolled Keywords: | bse,m2b cell,mouse bioassay,prion,prp,strain characterization,biochemistry,cellular and molecular neuroscience,cell biology,infectious diseases,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/1300/1303 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 02 Dec 2025 15:30 |
| Last Modified: | 18 Jun 2026 20:48 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/101201 |
| DOI: | 10.1080/19336896.2017.1331809 |
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