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ToolsSuh, Tae Young, Roh, In Soon, Kim, Hyo Jin, Griffiths, Peter C., Park, Kyung Je, Park, Hoo Chang, Hope, James, Kang, Hae Eun, Kim, Dae Yong and Sohn, Hyun Joo (2017) Biological and biochemical characterization of M2B cells:Classical BSE prion is conserved in transgenic mice overexpressing bovine prion protein gene. Prion, 11 (6). pp. 405-414. ISSN 1933-6896
Full text not available from this repository. (Request a copy)Abstract
M2B cells with persistent classical bovine spongiform encephalopathy (C-BSE) have been established previously. In this study, we performed strain characterization of the M2B cell line in bovine PrPC overexpressing mice (Tg 1896). Mice intracranially inoculated with M2B cells and C-BSE survived for 451 ± 7 and 465 ± 31 d post inoculation, respectively. Although biochemical properties, including deglycosylation and conformational stability, differed between M2B cells and C-BSE, inoculation with M2B cell lysate and C-BSE resulted in comparable phenotypes. Comparable vacuolation scores and PrPSc depositions were observed in the brain of Tg 1896 inoculated with both M2B cell lysate and C-BSE. Our results show that biochemical and biological characteristics of M2B cells and C-BSE are classifiable in the same strain.
| Item Type: | Article |
|---|---|
| Additional Information: | Publisher Copyright: © 2017 Crown copyright and Animal and Plant Quarantine Agency. |
| Uncontrolled Keywords: | bse,m2b cell,mouse bioassay,prion,prp,strain characterization,biochemistry,cellular and molecular neuroscience,cell biology,infectious diseases,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/1300/1303 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 02 Dec 2025 15:30 |
| Last Modified: | 08 Dec 2025 12:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/101201 |
| DOI: | 10.1080/19336896.2017.1331809 |
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