129/Ola mice carrying a null mutation in PrP that abolishes mRNA production are developmentally normal

Manson, J. C., Clarke, A. R., Hooper, M. L., Aitchison, L., McConnell, I. and Hope, J. (1994) 129/Ola mice carrying a null mutation in PrP that abolishes mRNA production are developmentally normal. Molecular Neurobiology, 8 (2-3). pp. 121-127. ISSN 0893-7648

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Abstract

The neural membrane glycoprotein PrP is implicated in the pathogenesis of the transmissible spongiform encephalopathies; however, the normal function of PrP and its precise role in disease are not understood. Recently, gene targeting has been used to produce mice with neo/PrP fusion transcripts, but no detectable PrP protein in the brain (1). Here we report the use of a different targeting strategy, to produce inbred mice with a complete absence of both PrP protein and mRNA sequences. At 7 mo of age, these mice show no overt phenotypic abnormalities despite the normal high levels of expression of PrP during mouse development. The mice are being used in experiments designed to address the role of PrP in the pathogenesis of scrapie and the replication of infectivity.

Item Type:	Article
Uncontrolled Keywords:	gene targeting,null mutation,prp gene,neuroscience (miscellaneous),neurology,cellular and molecular neuroscience ,/dk/atira/pure/subjectarea/asjc/2800/2801
Faculty \ School:	Faculty of Science > School of Biological Sciences
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	02 Dec 2025 15:30
Last Modified:	10 Dec 2025 14:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/101191
DOI:	10.1007/BF02780662

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