Infrared spectroscopic profiling of volatile metabolites from uropathogenic bacteria: Basic investigations toward rapid UTI diagnostics

Maiti, Kiran Sankar, Roy, Susmita, Zenner, Christian, Hall, Lindsay J., Hauer, Jürgen and Sroka, Ronald (2025) Infrared spectroscopic profiling of volatile metabolites from uropathogenic bacteria: Basic investigations toward rapid UTI diagnostics. Analytical Chemistry, 97 (39). pp. 21449-21458. ISSN 0003-2700

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Abstract

Urinary tract infections (UTIs) are among the most common bacterial infections, typically begin in the urethra but capable of rapidly progressing to the bladder and kidneys. Without timely intervention, these infections can lead to renal failure and, in severe cases, multiorgan failure. Current diagnostic methods for identifying bacterial pathogens are often time-consuming, highlighting the need for faster, more efficient detection techniques. Metabolite-based bacterial diagnostics offer a promising alternative, enabling minimally or noninvasive detection without the need for bacterial culture─provided that the metabolic profiles of individual bacterial strains are well characterized. This study investigates ten bacterial species associated with UTIs through the analysis of their volatile metabolites using Fourier-transform infrared (FT-IR) spectroscopy. Numerous spectral features corresponding to distinct metabolites were identified. Within a multidimensional metabolic space, each bacterial strain exhibited a unique volatile metabolite profile, serving as the basis for accurate identification. This approach lays the groundwork for future diagnostic platforms in which FT-IR spectroscopy could serve as a rapid, culture-free analytical tool to detect bacterial pathogens directly from exhaled breath and/or urine.

Item Type:	Article
Additional Information:	Funding information: We acknowledge Horst-Jürgen-Lühl Stiftung for financial support. J.H. and K.S.M. acknowledge funding by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)-547756633.
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Related URLs:	https://pubs.acs.org/doi/10.1021/acs.ana...
Depositing User:	LivePure Connector
Date Deposited:	07 Oct 2025 13:30
Last Modified:	07 Oct 2025 13:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/100665
DOI:	10.1021/acs.analchem.5c03269

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