Transcriptome sequencing of Festulolium accessions under salt stress

Teshome, A., Byrne, S. L., Didion, T., De Vega, J., Jensen, C. S., Klaas, M. and Barth, S. (2019) Transcriptome sequencing of Festulolium accessions under salt stress. BMC Research Notes, 12. ISSN 1756-0500

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Abstract

Objectives: The objective of this study was to establish transcriptome assemblies of Festulolium hybrids under salt stress, and identify genes regulated across the hybrids in response to salt stress. The development of transcriptome assemblies for Festulolium hybrids and cataloguing of genes regulated under salt stress will facilitate further downstream studies. Results: Plants were grown at three salt concentrations (0.5%, 1% and 1.5%) and phenotypic and transcriptomic data was collected. Salt stress was confirmed by progressive loss of green leaves as salt concentration increased from 0 to 1.5%. We generated de-novo transcriptome assemblies for two Festulolium pabulare festucoid genotypes, for a single Festulolium braunii genotype, and a single F. pabulare loloid genotype. We also identified 1555 transcripts that were up regulated and 1264 transcripts that were down regulated in response to salt stress in the Festulolium hybrids. Some of the identified transcripts showed significant sequence similarity with genes known to be regulated during salt and other abiotic stresses.

Item Type:	Article
Additional Information:	Availability of data and materials: The transcriptomic data have been deposited: ArrayExpress accession E-MTAB-7720. Funding information: This project has been funded through the FP7 Grant GrassMargins (FP7-KBBE-2011-5-289461). AT was supported by Marie Sklodowska-Curie Actions COFUND CAROLINE Grant Agreement no. CLNE/2017/364. The funding body had no role to play in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.
Uncontrolled Keywords:	festulolium,salt tolerance,transcriptome,biochemistry, genetics and molecular biology(all) ,/dk/atira/pure/subjectarea/asjc/1300
Faculty \ School:	Faculty of Science > School of Biological Sciences
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	02 Sep 2025 14:30
Last Modified:	02 Sep 2025 14:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/100271
DOI:	10.1186/s13104-019-4349-2

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