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ToolsTomaszewska, Paulina, Pellny, Till K., Hernández, Luis M., Mitchell, Rowan A. C., Castiblanco, Valheria, de Vega, José J., Schwarzacher, Trude and Heslop-Harrison, Pat J. S. (2021) Flow cytometry-based determination of ploidy from dried leaf specimens in genomically complex collections of the tropical forage grass urochloa s. l. Genes, 12 (7). ISSN 2073-4425
Full text not available from this repository. (Request a copy)Abstract
Urochloa (including Brachiaria, Megathyrus and some Panicum) tropical grasses are native to Africa and are now, after selection and breeding, planted worldwide, particularly in South America, as important forages with huge potential for further sustainable improvement and conservation of grasslands. We aimed to develop an optimized approach to determine ploidy of germplasm collection of this tropical forage grass group using dried leaf material, including approaches to collect, dry and preserve plant samples for flow cytometry analysis. Our methods enable robust identification of ploidy levels (coefficient of variation of G0/G1 peaks, CV, typically <5%). Ploidy of some 348 forage grass accessions (ploidy range from 2x to 9x), from international genetic resource collections, showing variation in basic chromosome numbers and reproduction modes (apomixis and sexual), were determined using our defined standard protocol. Two major Urochloa agamic complexes are used in the current breeding programs at CIAT and EMBRAPA: the ’brizantha’ and ’humidicola’ agamic complexes are variable, with multiple ploidy levels. Some U. brizantha accessions have odd level of ploidy (5x), and the relative differences in fluorescence values of the peak positions between adjacent cytotypes is reduced, thus more precise examination of this species is required. Ploidy measurement of U. humidicola revealed aneuploidy.
| Item Type: | Article |
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| Additional Information: | Data Availability Statement: Not applicable. Funding information: This work was supported under the RCUK-CIAT Newton-Caldas Initiative “Exploiting biodiversity in Brachiaria and Panicum tropical forage grasses using genetics to improve livelihoods and sustainability”, with funding from UK’s Official Development Assistance Newton Fund awarded by UK Biotechnology and Biological Sciences Research Council (BB/R022828/1). P.T. has received further support from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreements No 844564 and No 101006417. Open access charge was financially supported by the Leicester Research Archive, University of Leicester. |
| Uncontrolled Keywords: | apomixis,brachiaria,dried specimens,flow cytometry,panicum,ploidy,tropical forage grasses,genetics,genetics(clinical) ,/dk/atira/pure/subjectarea/asjc/1300/1311 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 21 Aug 2025 15:30 |
| Last Modified: | 21 Aug 2025 15:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/100202 |
| DOI: | 10.3390/genes12070957 |
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