Molecularly imprinted polymer-coated quantum dots for multiplexed cell targeting and imaging

Panagiotopoulou, Maria, Salinas, Yolanda, Beyazit, Selim, Kunath, Stephanie, Duma, Luminita, Prost, Elise, Mayes, Andrew G., Resmini, Marina, Tse Sum Bui, Bernadette and Haupt, Karsten (2016) Molecularly imprinted polymer-coated quantum dots for multiplexed cell targeting and imaging. Angewandte Chemie International Edition, 55 (29). pp. 8244-8248. ISSN 1433-7851

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    Advanced tools for cell imaging are of great interest to detect, localize and quantify molecular biomarkers of cancer or infection. We describe a novel photopolymerization method to coat quantum dots (QDs) with polymer shells, in particular molecularly imprinted polymers (MIPs), using the visible light emitted from QDs excited by UV light. Fluorescent core-shell particles specifically recognizing glucuronic acid (GlcA) or N-acetylneuraminic acid (NANA) were prepared. Simultaneous multiplexed labeling of human keratinocytes with green QDs conjugated with MIP-GlcA and red QDs conjugated with MIP-NANA was demonstrated by fluorescence imaging. The specificity of binding was verified with a non-imprinted control polymer and by enzymatically cleaving the terminal GlcA and NANA moieties. The coating strategy is potentially a generic method to functionalize QDs to address a much wider range of biocompatibility and biorecognition issues.

    Item Type: Article
    Uncontrolled Keywords: bioimaging,synthetic receptor,quantum dot,molecularly imprinted polymer,nanocomposite
    Faculty \ School: Faculty of Science > School of Chemistry
    Depositing User: Pure Connector
    Date Deposited: 27 May 2016 17:00
    Last Modified: 24 May 2019 00:46
    DOI: 10.1002/ange.201601122

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