Cautionary notes on the use of C-terminal BAK1 fusion proteins for functional studies

Ntoukakis, Vardis, Schwessinger, Benjamin, Segonzac, Cécile and Zipfel, Cyril (2011) Cautionary notes on the use of C-terminal BAK1 fusion proteins for functional studies. The Plant Cell, 23 (11). pp. 3871-3878. ISSN 1040-4651

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Abstract

Detailed phenotypic characterization reveals that several BAK1 fusion proteins with C-terminal tags strongly impair complementation of bak1 null mutants with respect to responsiveness to the bacterial pathogen-associated molecular patterns flagellin and EF-Tu. This raises concerns about the widespread use of such protein variants of this important regulatory Leu-rich repeat receptor-like kinase (RLK) for functional analyses of RLK-based signaling.

Item Type: Article
Uncontrolled Keywords: arabidopsis,arabidopsis proteins,brassinosteroids,flagellin,mutation,peptide elongation factor tu,plants, genetically modified,protein kinases,protein-serine-threonine kinases,recombinant fusion proteins,signal transduction
Faculty \ School: Faculty of Science > School of Biological Sciences
University of East Anglia > Faculty of Science > Research Groups > Plant Sciences
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Depositing User: Pure Connector
Date Deposited: 04 Jul 2014 13:44
Last Modified: 25 Jul 2018 09:44
URI: https://ueaeprints.uea.ac.uk/id/eprint/48875
DOI: 10.1105/tpc.111.090779

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