NAD+-dependent DNA ligases of Mycobacterium tuberculosis and Streptomyces coelicolor.

Wilkinson, A, Sayer, H, Bullard, D, Smith, A, Day, J, Kieser, T and Bowater, R (2003) NAD+-dependent DNA ligases of Mycobacterium tuberculosis and Streptomyces coelicolor. Proteins: Structure Function and Bioinformatics, 51 (3). pp. 321-326. ISSN 0887-3585

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    Abstract

    Sequencing of the genomes of Mycobacterium tuberculosis H37Rv and Streptomyces coelicolor A3(2) identified putative genes for an NAD+-dependent DNA ligase. We have cloned both open reading frames and overexpressed the protein products in Escherichia coli. In vitro biochemical assays confirm that each of these proteins encodes a functional DNA ligase that uses NAD+ as its cofactor. Expression of either protein is able to complement E. coli GR501, which carries a temperature-sensitive mutation in ligA. Thus, in vitro and in vivo analyses confirm predictions that ligA genes from M. tuberculosis and S. coelicolor are NAD+-dependent DNA ligases.

    Item Type: Article
    Faculty \ School: Faculty of Science > School of Biological Sciences
    University of East Anglia > Faculty of Science > Research Groups > Molecular Microbiology
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    Depositing User: EPrints Services
    Date Deposited: 01 Oct 2010 14:37
    Last Modified: 25 Jul 2018 07:01
    URI: https://ueaeprints.uea.ac.uk/id/eprint/463
    DOI: 10.1002/prot.10361

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