Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.

Chan, SSM, McConnell, I and Blacklaws, BA (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes. Immunology, 107 (3). pp. 366-372. ISSN 0019-2805

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Abstract

Dendritic cells (DCs) are professional antigen-presenting cells with a highly immunostimulatory function and the capacity to activate naïve T cells. In recent years the rapid progress in mouse and human DC research can be mainly attributed to the generation of DCs from precursor cells in vitro, although a lack of reagents has hampered DC research in many large animal models. Here we describe the generation and characterization of ovine monocyte-derived DCs in vitro. In addition to the characteristic morphology and non-adherence of DCs, peripheral blood mononuclear cell monocytes cultured with ovine granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) expressed CD11c and major histocompatibility complex (MHC) class II, but did not express CD14. High levels of endocytosis and an ability to stimulate antigen-specific proliferation of CD4 T lymphocytes were also demonstrated.

Item Type: Article
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
University of East Anglia > Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
Depositing User: Deborah Clemitshaw
Date Deposited: 28 Mar 2013 22:16
Last Modified: 25 Jul 2018 07:17
URI: https://ueaeprints.uea.ac.uk/id/eprint/42058
DOI:

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