Reusable Surface Plasmon Resonance Assay for the Specific Detection of Streptococcus Pneumoniae tmRNA.

Glynn, B, Lacey, K, O'Grady, Justin, Barry, T, Smith, TJ and Maher, M (2008) Reusable Surface Plasmon Resonance Assay for the Specific Detection of Streptococcus Pneumoniae tmRNA. Journal of Rapid Methods & Automation in Microbiology, 16 (3). pp. 210-221. ISSN 1745-4581

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Abstract

A capture oligonucleotide-based surface plasmon resonance assay was developed for the specific detection of tmRNA from the gram-positive pathogen Streptococcus pneumoniae. Candidate species-specific probes were initially screened on microarrays using labeled in vitro transcribed tmRNA beforebeing subjected to further analysis on a Biacore 2000 biosensor using a variety of unlabeled tmRNA-containing samples. Previous work had demonstrated that there is approximately 1,000 copies/cfu of tmRNA in S. pneumoniae. The natural amplification of this target has the potential to improve the detection capability of a nucleic acid diagnostics assay. In this study, S. pneumoniae-specific probes were designed that enabled the detection of 10 exp12 cRNA copies of the tmRNA target on the Biacore instrument. When evaluated in combination with nucleic acid sequence-based amplification, specific detection of tmRNA from a single S. pneumoniae cell was demonstrated.

Item Type: Article
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Related URLs:
Depositing User: Sophie Buckingham
Date Deposited: 05 Mar 2013 10:25
Last Modified: 03 Jan 2019 10:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/41789
DOI: 10.1111/j.1745-4581.2008.00128.x

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