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Radford, David S., Kihlken, Margaret A., Borrelly, Gilles P. M., Harwood, Colin R., Le Brun, Nick E. 
ORCID: https://orcid.org/0000-0001-9780-4061 and Cavet, Jennifer S.
(2003)
CopZ from Bacillus subtilis interacts in vivo with a copper exporting CPx-type ATPase CopA.
FEMS Microbiology Letters, 220 (1).
pp. 105-112.
ISSN 0378-1097
Abstract
The structure of the hypothetical copper-metallochaperone CopZ from Bacillus subtilis and its predicted partner CopA have been studied but their respective contributions to copper export, -import, -sequestration and -supply are unknown. ΔcopA was hypersensitive to copper and contained more copper atoms cell-1 than wild-type. Expression from the copA operator-promoter increased in elevated copper (not other metals), consistent with a role in copper export. A bacterial two-hybrid assay revealed in vivo interaction between CopZ and the N-terminal domain of CopA but not that of a related transporter, YvgW, involved in cadmium-resistance. Activity of copper-requiring cytochrome caa3 oxidase was retained in ΔcopZ and ΔcopA. ΔcopZ was only slightly copper-hypersensitive but ΔcopZ/ΔcopA was more sensitive than ΔcopA, implying some action of CopZ that is independent of CopA. Significantly, ΔcopZ contained fewer copper atoms cell-1 than wild-type under these conditions. CopZ makes a net contribution to copper sequestration and/or recycling exceeding any donation to CopA for export.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | atx1,copa,copz,cytochrome oxidase,metallochaperone,p-type atpase,microbiology,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/2400/2404 |
| Faculty \ School: | Faculty of Science > School of Chemistry (former - to 2024) |
| UEA Research Groups: | Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Chemistry of Life Processes (former - to 2025) Faculty of Science > Research Groups > Pharmacology and Biological Chemistry |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 18 Jun 2025 09:30 |
| Last Modified: | 18 Jun 2026 20:39 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/99601 |
| DOI: | 10.1016/S0378-1097(03)00095-8 |
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