Modification of bacterial microcompartments with target biomolecules via post-translational SpyTagging

Beal, David M., Liang, Mingzhi, Brown, Ian, Budge, James D., Burrows, Emily R., Howland, Kevin, Lee, Phoebe, Martin, Sarah, Morrell, Andrew, Nemoto-Smith, Emi, Roobol, Joanne, Stanley, Maria, Smales, C. Mark and Warren, Martin J. ORCID: (2023) Modification of bacterial microcompartments with target biomolecules via post-translational SpyTagging. Materials Advances, 4 (14). pp. 2963-2970. ISSN 2633-5409

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Bacterial microcompartments (BMCs) are proteinaceous organelle-like structures formed within bacteria, often encapsulating enzymes and cellular processes, in particular, allowing toxic intermediates to be shielded from the general cellular environment. Outside of their biological role they are of interest, through surface modification, as potential drug carriers and polyvalent antigen display scaffolds. Here we use a post-translational modification approach, using copper free click chemistry, to attach a SpyTag to a target protein molecule for attachment to a specific SpyCatcher modified BMC shell protein. We demonstrate that a post-translationally SpyTagged material can react with a SpyCatcher modified BMC and show its presence on the surface of BMCs, enabling future investigation of these structures as polyvalent antigen display scaffolds for vaccine development. This post-translational ‘click’ methodology overcomes the necessity to genetically encode the SpyTag, avoids any potential reduction in expression yield and expands the scope of SpyTag/SpyCatcher vaccine scaffolds to form peptide epitope vaccines and small molecule delivery agents.

Item Type: Article
Additional Information: Funding information: This work was partially funded by BBSRC grant BB/V011324/1 (to CMS and MJW), Wellcome Trust Grant 209171/Z/17/Z (to DMB) and BBSRC grants BB/S014020/1 and BB/W014165/1 (to ML). The authors also thank the University of Kent for the award of funding to support the work.
Uncontrolled Keywords: chemistry (miscellaneous),materials science(all),sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/1600/1601
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Faculty of Science
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging
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Depositing User: LivePure Connector
Date Deposited: 02 Jul 2024 19:31
Last Modified: 17 Jul 2024 01:46
DOI: 10.1039/D3MA00071K


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