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Mahbubani, Hiro M., Chong, James P. J., Chevalier, Stephane, Thömmes, Pia and Blow, J. Julian 
ORCID: https://orcid.org/0000-0002-9524-5849
(1997)
Cell cycle regulation of the replication licensing system: involvement of a Cdk-dependent inhibitor.
Journal of Cell Biology, 136 (1).
pp. 125-135.
ISSN 0021-9525
Abstract
The replication licensing factor (RLF) is an essential initiation factor that is involved in preventing re-replication of chromosomal DNA in a single cell cycle. In Xenopus egg extracts, it can be separated into two components: RLF-M, a complex of MCM/P1 polypeptides, and RLF-B, which is currently unpurified. In this paper we investigate variations in RLF activity throughout the cell cycle. Total RLF activity is low in metaphase, due to a lack of RLF-B activity and the presence of an RLF inhibitor. RLF-B is rapidly activated on exit from metaphase, and then declines during interphase. The RLF inhibitor present in metaphase extracts is dependent on the activity of cyclin-dependent kinases (Cdks). Affinity depletion of Cdks from metaphase extracts removed the RLF inhibitor, while Cdc2/cyclin B directly inhibited RLF activity. In metaphase extracts treated with the protein kinase inhibitor 6-dimethylaminopurine (6-DMAP), both cyclin B and the RLF inhibitor were stabilized although the extracts morphologically entered interphase. These results are consistent with studies in other organisms that invoke a key role for Cdks in preventing re-replication of DNA in a single cell cycle.
| Item Type: | Article |
|---|---|
| Faculty \ School: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 11 Jun 2024 09:30 |
| Last Modified: | 18 Jun 2026 20:14 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/95541 |
| DOI: | 10.1083/jcb.136.1.125 |
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