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Benatti, Paolo, Belluti, Silvia, Miotto, Benoit, Neusiedler, Julia, Dolfini, Diletta, Drac, Marjorie, Basile, Valentina, Schwob, Etienne, Mantovani, Roberto, Blow, J. Julian 
ORCID: https://orcid.org/0000-0002-9524-5849 and Imbriano, Carol
(2016)
Direct non transcriptional role of NF-Y in DNA replication.
Biochimica Et Biophysica Acta-Molecular Cell Research, 1863 (4).
pp. 673-685.
ISSN 0167-4889
Abstract
NF-Y is a heterotrimeric transcription factor, which plays a pioneer role in the transcriptional control of promoters containing the CCAAT-box, among which genes involved in cell cycle regulation, apoptosis and DNA damage response. The knock-down of the sequence-specific subunit NF-YA triggers defects in S-phase progression, which lead to apoptotic cell death. Here, we report that NF-Y has a critical function in DNA replication progression, independent from its transcriptional activity. NF-YA colocalizes with early DNA replication factories, its depletion affects the loading of replisome proteins to DNA, among which Cdc45, and delays the passage from early to middle-late S phase. Molecular combing experiments are consistent with a role for NF-Y in the control of fork progression. Finally, we unambiguously demonstrate a direct non-transcriptional role of NF-Y in the overall efficiency of DNA replication, specifically in the DNA elongation process, using a Xenopus cell-free system. Our findings broaden the activity of NF-Y on a DNA metabolism other than transcription, supporting the existence of specific TFs required for proper and efficient DNA replication.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ccaat-binding factor,dna replication,nf-y,transcription factors,xenopus cell-free system |
| Faculty \ School: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 10 Jun 2024 15:31 |
| Last Modified: | 23 Jul 2024 02:22 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/95469 |
| DOI: | 10.1016/j.bbamcr.2015.12.019 |
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