Dynamic SUMO modification regulates mitotic chromosome assembly and cell cycle progression in Caenorhabditis elegans

Pelisch, Federico, Sonneville, Remi, Pourkarimi, Ehsan, Agostinho, Ana, Blow, J. Julian, Gartner, Anton and Hay, Ronald T. (2014) Dynamic SUMO modification regulates mitotic chromosome assembly and cell cycle progression in Caenorhabditis elegans. Nature Communications, 5. ISSN 2041-1723

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Abstract

The small ubiquitin-like modifier (SUMO), initially characterized as a suppressor of a mutation in the gene encoding the centromeric protein MIF2, is involved in many aspects of cell cycle regulation. The dynamics of conjugation and deconjugation and the role of SUMO during the cell cycle remain unexplored. Here we used Caenorhabditis elegans to establish the contribution of SUMO to a timely and accurate cell division. Chromatin-associated SUMO conjugates increase during metaphase but decrease rapidly during anaphase. Accumulation of SUMO conjugates on the metaphase plate and proper chromosome alignment depend on the SUMO E2 conjugating enzyme UBC-9 and SUMO E3 ligase PIAS(GEI-17). Deconjugation is achieved by the SUMO protease ULP-4 and is crucial for correct progression through the cell cycle. Moreover, ULP-4 is necessary for Aurora B(AIR-2) extraction from chromatin and relocation to the spindle mid-zone. Our results show that dynamic SUMO conjugation plays a role in cell cycle progression.

Item Type: Article
Additional Information: Funding information: This work was supported by the Wellcome Trust (grants 098391/Z/12/7 to R.T.H., 090944/Z/09/Z to A.G. and 097945/B/11/Z to J.J.B.), Cancer Research UK (grants C434/A13067 to R.T.H. and C303/A7399 to J.J.B.), and the BBSRC (grant BB/J015199/1 to A.G. and R.T.H). We acknowledge the Light Microscopy Facility, College of Life Sciences, University of Dundee (supported by Wellcome Trust Technology Platform grant 097945/B/11/Z). F.P. is the recipient of a Marie Curie Fellowship (grant agreement 297881) and is a career investigator of the Argentinean research council (CONICET), also supported by a fellowship from CONICET (Res. N° 4771/12). Some nematode strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). A.G. is a Wellcome Trust Senior Biomedical Research Fellow. R.T.H. and J.J.B. are Senior Investigators of the Wellcome Trust.
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Depositing User: LivePure Connector
Date Deposited: 10 Jun 2024 09:30
Last Modified: 10 Jun 2024 09:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/95424
DOI: 10.1038/ncomms6485

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