A small predatory core genome in the divergent marine Bacteriovorax marinus SJ and the terrestrial Bdellovibrio bacteriovorus

Crossman, Lisa C., Chen, Huan, Cerdeño-Tárraga, Ana M., Brooks, Karen, Quail, Michael A., Pineiro, Silvia A., Hobley, Laura, Sockett, R. Elizabeth, Bentley, Stephen D., Parkhill, Julian, Williams, Henry N. and Stine, O. Colin (2013) A small predatory core genome in the divergent marine Bacteriovorax marinus SJ and the terrestrial Bdellovibrio bacteriovorus. ISME Journal, 7 (1). pp. 148-160. ISSN 1751-7362

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Bacteriovorax marinus SJ is a predatory delta-proteobacterium isolated from a marine environment. The genome sequence of this strain provides an interesting contrast to that of the terrestrial predatory bacterium Bdellovibrio bacteriovorus HD100. Based on their predatory lifestyle, Bacteriovorax were originally designated as members of the genus Bdellovibrio but subsequently were re-assigned to a new genus and family based on genetic and phenotypic differences. B. marinus attaches to Gram-negative bacteria, penetrates through the cell wall to form a bdelloplast, in which it replicates, as shown using microscopy. Bacteriovorax is distinct, as it shares only 30% of its gene products with its closest sequenced relatives. Remarkably, 34% of predicted genes over 500 nt in length were completely unique with no significant matches in the databases. As expected, Bacteriovorax shares several characteristic loci with the other delta-proteobacteria. A geneset shared between Bacteriovorax and Bdellovibrio that is not conserved among other delta-proteobacteria such as Myxobacteria (which destroy prey bacteria externally via lysis), or the non-predatory Desulfo-bacteria and Geobacter species was identified. These 291 gene orthologues common to both Bacteriovorax and Bdellovibrio may be the key indicators of host-interaction predatory-specific processes required for prey entry. The locus from Bdellovibrio bacteriovorus is implicated in the switch from predatory to prey/host-independent growth. Although the locus is conserved in B. marinus, the sequence has only limited similarity. The results of this study advance understanding of both the similarities and differences between Bdellovibrio and Bacteriovorax and confirm the distant relationship between the two and their separation into different families.

Item Type: Article
Additional Information: Funding Information: Sequencing and annotation of Bacteriovorax marinus SJ was funded by the Wellcome Trust. Microscopy was supported by National Science Foundation award HRD 0932137 to HW and by BBSRC UK G003092/1 to RES. LC is funded by BBSRC in partnership with East of England Development Agency, Norfolk County, Norwich City and South Norfolk Councils and the Greater Norwich Development Partnership, UK. The complete manually annotated chromosome of Bacteriovorax marinus SJ is available at FQ312005; plasmid pBMS1 has been assigned accession number HE610506.
Uncontrolled Keywords: bacteriovorax,balo,bdellovibrio,genome sequence,host-interaction locus,subtractive hybridization,microbiology,ecology, evolution, behavior and systematics,sdg 14 - life below water ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 14 May 2024 09:33
Last Modified: 20 May 2024 02:32
URI: https://ueaeprints.uea.ac.uk/id/eprint/95146
DOI: 10.1038/ismej.2012.90

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