Alternative dimethylsulfoniopropionate biosynthesis enzymes in diverse and abundant microbes

Wang, Jinyan, Curson, Andrew, Zhou, Shun, Carrion Fonseca, Ornella, Liu, Ji, Vieira, Ana, Walsham, Keanu, Monaco, Serena, Li, Chun-Yang, Dong, Qing-Yu, Wang, Yu, Rivera, Peter, Wang, Xiao-Di, Zhang, Min, Hanwell, Libby, Wallace, Matthew ORCID: https://orcid.org/0000-0002-5751-1827, Zhu, Xiaoyu, Leao, Pedro, Lea-Smith, David ORCID: https://orcid.org/0000-0003-2463-406X, Zhang, Yu-Zhong, Zhang, Xiao-Hua and Todd, Jonathan (2024) Alternative dimethylsulfoniopropionate biosynthesis enzymes in diverse and abundant microbes. Nature Microbiology. ISSN 2058-5276 (In Press)

Microsoft Word (OpenXML) (NMICROBIOL-23010222-tracked changes) - Accepted Version Restricted to Repository staff only until 31 December 2099. Request a copy

Abstract

Dimethylsulfoniopropionate (DMSP) is an abundant marine organosulfur compound with roles in stress protection, chemotaxis, nutrient and sulfur cycling and climate regulation. Here, we report the discovery of a bifunctional DMSP biosynthesis enzyme, DsyGD, in the transamination pathway of the rhizobacterium Gynuella sunshinyii and some filamentous cyanobacteria not previously known to produce DMSP. DsyGD produces DMSP through its N-terminal DsyG methylthiohydroxybutyrate (MTHB) S-methyltransferase and C-terminal DsyD dimethylsulfoniohydroxybutyrate (DMSHB) decarboxylase domains. Phylogenetically distinct DsyG-like proteins, termed DSYE, with MTHB S-methyltransferase (MSM) activity were found in diverse and environmentally abundant algae, comprising a mix of low, high and previously unknown DMSP producers. Algae containing DSYE, particularly bloom-forming Pelagophyceae species, were globally more abundant DMSP producers than those with previously described DMSP synthesis genes. This work greatly increases the number and diversity of predicted DMSP-producing organisms and highlights the importance of Pelagophyceae and other DSYE-containing algae in global DMSP production and sulfur cycling.

Item Type:	Article
Uncontrolled Keywords:	sdg 13 - climate action,sdg 14 - life below water ,/dk/atira/pure/sustainabledevelopmentgoals/climate_action
Faculty \ School:	Faculty of Science > School of Biological Sciences University of East Anglia Research Groups/Centres > Theme - ClimateUEA Faculty of Science Faculty of Science > School of Pharmacy
UEA Research Groups:	Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Groups > Pharmaceutical Materials and Soft Matter
Depositing User:	LivePure Connector
Date Deposited:	26 Apr 2024 14:31
Last Modified:	29 Apr 2024 12:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/95031
DOI:

Actions (login required)

View Item