Genetic and structural diversity of prokaryotic ice-binding proteins from the central Arctic Ocean

Winder, Johanna C. ORCID:, Boulton, William, Salamov, Asaf, Eggers, Sarah Lena, Metfies, Katja, Moulton, Vincent ORCID: and Mock, Thomas ORCID: (2023) Genetic and structural diversity of prokaryotic ice-binding proteins from the central Arctic Ocean. Genes, 14 (2). ISSN 2073-4425

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Ice-binding proteins (IBPs) are a group of ecologically and biotechnologically relevant enzymes produced by psychrophilic organisms. Although putative IBPs containing the domain of unknown function (DUF) 3494 have been identified in many taxa of polar microbes, our knowledge of their genetic and structural diversity in natural microbial communities is limited. Here, we used samples from sea ice and sea water collected in the central Arctic Ocean as part of the MOSAiC expedition for metagenome sequencing and the subsequent analyses of metagenome-assembled genomes (MAGs). By linking structurally diverse IBPs to particular environments and potential functions, we reveal that IBP sequences are enriched in interior ice, have diverse genomic contexts and cluster taxonomically. Their diverse protein structures may be a consequence of domain shuffling, leading to variable combinations of protein domains in IBPs and probably reflecting the functional versatility required to thrive in the extreme and variable environment of the central Arctic Ocean.

Item Type: Article
Additional Information: Funding Information: W.B. was supported by the Natural Environment Research Council and ARIES DTP [grant number NE/S007334/1]. J.C.W. was supported by the UKRI Biotechnology and Biological Sciences Research Council Norwich Research Park Biosciences Doctoral Training Partnership [grant number BB/T008717/1]. The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. Data used in this manuscript were produced as part of the international Multidisciplinary drifting Observatory for the Study of the Arctic Climate (MOSAiC) with the tag MOSAiC20192020 (expedition AWI_PS_122_00). This work was also supported through the Research Council of Norway through project HAVOC (grant no 280292), and the National Science Foundation through grant OPP-1735862. Publisher Copyright: © 2023 by the authors.
Uncontrolled Keywords: arctic ocean,domain shuffling,duf3494,ice-binding proteins,mags,metagenomics,mosaic expedition,polar genomics,genetics,genetics(clinical) ,/dk/atira/pure/subjectarea/asjc/1300/1311
Faculty \ School: Faculty of Science > School of Environmental Sciences
Faculty of Science > School of Computing Sciences
University of East Anglia Research Groups/Centres > Theme - ClimateUEA
UEA Research Groups: Faculty of Science > Research Groups > Norwich Epidemiology Centre
Faculty of Medicine and Health Sciences > Research Groups > Norwich Epidemiology Centre
Faculty of Science > Research Groups > Computational Biology
Faculty of Science > Research Centres > Centre for Ecology, Evolution and Conservation
Faculty of Science > Research Groups > Centre for Ocean and Atmospheric Sciences
Faculty of Science > Research Groups > Environmental Biology
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Depositing User: LivePure Connector
Date Deposited: 11 Mar 2024 10:31
Last Modified: 25 Mar 2024 17:30
DOI: 10.3390/genes14020363


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