Frustule-related gene transcription and the influence of diatom community composition on silica precipitation in an iron-limited environment

Durkin, Colleen A., Marchetti, Adrian, Bender, Sara J., Truong, Tiffany, Morales, Rhonda, Mock, Thomas ORCID: and Armbrust, E. Virginia (2012) Frustule-related gene transcription and the influence of diatom community composition on silica precipitation in an iron-limited environment. Limnology and Oceanography, 57 (6). pp. 1619-1633. ISSN 0024-3590

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A microcosm study in iron-limited waters of the northeast subarctic Pacific Ocean was conducted to examine how iron availability affects the frustule-related response of individual diatoms and thus the total quantity of silica precipitated by the community. New silica precipitated per cell was estimated using the fluorescent cell stain 2-(4-pyridyl)-5{[4-dimethylaminoethyl-aminocarbamoyl)-methoxy]phenyl}oxazole (PDMPO). Differences in new silica precipitation within a particular genus before and after iron enrichment were small compared to differences among genera, indicating that the quantity of total silica precipitated is particularly sensitive to community composition. Transcriptional patterns of genes encoding silicon transporters, aminopropyltransferases, chitin synthases, and a protein with uncharacterized function were measured in natural populations to identify indicators of the frustule-related responses of different genera to iron limitation. Transcripts associated with silicon transporters were the most readily detectable in three metatranscriptome datasets and were capable of resolving species composition shifts and physiological responses. Silicon transporter transcripts from a distinct phylogenetic clade were most abundant in the iron-limited community, and transcripts from a separate clade were more abundant in the community that bloomed after iron enrichment. Transcripts of the gene present in the ironlimited community were also more abundant in iron-limited laboratory cultures of Pseudo-nitzschia multiseries, suggesting that this gene plays a role in silicon uptake during iron limitation. The responses of individual cells, as detected in this study, determine how the community influences silicon cycling in iron-limited environments.

Item Type: Article
Uncontrolled Keywords: oceanography,aquatic science ,/dk/atira/pure/subjectarea/asjc/1900/1910
Faculty \ School: Faculty of Science > School of Environmental Sciences
UEA Research Groups: Faculty of Science > Research Groups > Centre for Ocean and Atmospheric Sciences
Faculty of Science > Research Groups > Environmental Biology
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Depositing User: LivePure Connector
Date Deposited: 08 Mar 2024 12:33
Last Modified: 08 Mar 2024 14:33
DOI: 10.4319/lo.2012.57.6.1619

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