Differential temporal release and lipoprotein loading in B. thetaiotaomicron bacterial extracellular vesicles

Juodeikis, Rokas, Martins, Carlo, Saalbach, Gerhard, Richardson, Jake, Koev, Todor ORCID: https://orcid.org/0000-0002-8218-9753, Baker, Dave J., Defernez, Marianne, Warren, Martin ORCID: https://orcid.org/0000-0002-6028-6456 and Carding, Simon R. (2024) Differential temporal release and lipoprotein loading in B. thetaiotaomicron bacterial extracellular vesicles. Journal of Extracellular Vesicles, 13 (1). ISSN 2001-3078

[thumbnail of J of Extracellular Vesicle - 2024 - Juodeikis - Differential temporal release and lipoprotein loading in B]
Preview
PDF (J of Extracellular Vesicle - 2024 - Juodeikis - Differential temporal release and lipoprotein loading in B) - Published Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview

Abstract

Bacterial extracellular vesicles (BEVs) contribute to stress responses, quorum sensing, biofilm formation and interspecies and interkingdom communication. However, the factors that regulate their release and heterogeneity are not well understood. We set out to investigate these factors in the common gut commensal Bacteroides thetaiotaomicron by studying BEV release throughout their growth cycle. Utilising a range of methods, we demonstrate that vesicles released at different stages of growth have significantly different composition, with early vesicles enriched in specifically released outer membrane vesicles (OMVs) containing a larger proportion of lipoproteins, while late phase BEVs primarily contain lytic vesicles with enrichment of cytoplasmic proteins. Furthermore, we demonstrate that lipoproteins containing a negatively charged signal peptide are preferentially incorporated in OMVs. We use this observation to predict all Bacteroides thetaiotaomicron OMV enriched lipoproteins and analyse their function. Overall, our findings highlight the need to understand media composition and BEV release dynamics prior to functional characterisation and define the theoretical functional capacity of Bacteroides thetaiotaomicron OMVs.

Item Type: Article
Additional Information: Funding Information: The authors would like to thank Rik Haagmans for help with the bacteriophage assay and scientific support staff at Quadram Institute for providing general lab support for this research. We also thank George Savva for statistical analysis support. This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC) under Grant BB/R012490/1; BBSRC under Grant BB/CCG1860/1; BBSRC under Grant BB/S002197/1; and Royal Society under Grant INF/R2/180062.
Uncontrolled Keywords: bev,bacteroides,omv,bacterial extracellular vesicles,outer membrane vesicles,proteomics,histology,cell biology ,/dk/atira/pure/subjectarea/asjc/2700/2722
Faculty \ School: Faculty of Science > School of Biological Sciences
Faculty of Science > School of Pharmacy
Faculty of Medicine and Health Sciences > Norwich Medical School
Faculty of Science
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging
Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 24 Jan 2024 01:35
Last Modified: 31 Jan 2024 03:23
URI: https://ueaeprints.uea.ac.uk/id/eprint/94234
DOI: 10.1002/jev2.12406

Actions (login required)

View Item View Item