Targeted photodynamic therapy for breast cancer: the potential of glyconanoparticles

Thomas-Moore, Brydie A., Dedola, Simone, Russell, David A., Field, Robert A. and Marin, Maria J. ORCID: https://orcid.org/0000-0001-8021-5498 (2023) Targeted photodynamic therapy for breast cancer: the potential of glyconanoparticles. Nanoscale Advances, 5 (23). pp. 6501-6513. ISSN 2516-0230

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Abstract

Photodynamic therapy (PDT) uses a non-toxic light sensitive molecule, a photosensitiser, that releases cytotoxic reactive oxygen species upon activation with light of a specific wavelength. Here, glycan-modified 16 nm gold nanoparticles (glycoAuNPs) were explored for their use in targeted PDT, where the photosensitiser was localised to the target cell through selective glycan–lectin interactions. Polyacrylamide (PAA)-glycans were chosen to assess glycan binding to the cell lines. These PAA-glycans indicated the selective uptake of a galactose-derivative PAA by two breast cancer cell lines, SK-BR-3 and MDA-MD-231. Subsequently, AuNPs were modified with a galactose-derivative ligand and an amine derivate of the photosensitiser chlorin e6 was incorporated to the nanoparticle surface via amide bond formation using EDC/NHS coupling chemistry. The dual modified nanoparticles were investigated for the targeted cell killing of breast cancer cells, demonstrating the versatility of using glycoAuNPs for selective binding to different cancer cells and their potential use for targeted PDT.

Item Type: Article
Additional Information: Acknowledgements: This work was supported by Norwich Research Park Biotechnology and Biological Sciences Research Council (BBSRC) industrial CASE studentship in partnership with Iceni Glycoscience [reference BB/M016609/1]. The authors would like to thank Dr Paul Thomas (Henry Wellcome Lab for Cell Imaging, Faculty of Science, University of East Anglia) for his guidance on imaging techniques, and Dr Martin Rejzek and Dr Sergey A. Nepogodiev (John Innes Centre) for access to MS and NMR facilities, respectively. MJM would like to thank the School of Chemistry at the University of East Anglia for financial support. Graphical abstract was prepared using free templates from the “Library of Scientific & Medical illustration” (licence CC BY-NC-SA 4.0) http://www.somersault1824.com/.
Uncontrolled Keywords: engineering(all),bioengineering,atomic and molecular physics, and optics,chemistry(all),materials science(all),sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2200
Faculty \ School: Faculty of Science > School of Chemistry
UEA Research Groups: Faculty of Science > Research Groups > Centre for Photonics and Quantum Science
Faculty of Science > Research Groups > Chemistry of Materials and Catalysis
Faculty of Science > Research Groups > Chemistry of Light and Energy
Faculty of Science > Research Groups > Chemistry of Life Processes
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Depositing User: LivePure Connector
Date Deposited: 31 Oct 2023 02:43
Last Modified: 06 Dec 2023 02:20
URI: https://ueaeprints.uea.ac.uk/id/eprint/93511
DOI: 10.1039/D3NA00544E

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