Genome-scale sequencing and analysis of human, wolf, and bison DNA from 25,000-year-old sediment

Gelabert, Pere, Sawyer, Susanna, Bergström, Anders ORCID:, Margaryan, Ashot, Collin, Thomas C., Meshveliani, Tengiz, Belfer-Cohen, Anna, Lordkipanidze, David, Jakeli, Nino, Matskevich, Zinovi, Bar-Oz, Guy, Fernandes, Daniel M., Cheronet, Olivia, Özdoğan, Kadir T., Oberreiter, Victoria, Feeney, Robin N. M., Stahlschmidt, Mareike C., Skoglund, Pontus and Pinhasi, Ron (2021) Genome-scale sequencing and analysis of human, wolf, and bison DNA from 25,000-year-old sediment. Current Biology, 31 (16). 3564-3574.e9. ISSN 0960-9822

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Cave sediments have been shown to preserve ancient DNA but so far have not yielded the genome-scale information of skeletal remains. We retrieved and analyzed human and mammalian nuclear and mitochondrial environmental “shotgun” genomes from a single 25,000-year-old Upper Paleolithic sediment sample from Satsurblia cave, western Georgia:first, a human environmental genome with substantial basal Eurasian ancestry, which was an ancestral component of the majority of post-Ice Age people in the Near East, North Africa, and parts of Europe; second, a wolf environmental genome that is basal to extant Eurasian wolves and dogs and represents a previously unknown, likely extinct, Caucasian lineage; and third, a European bison environmental genome that is basal to present-day populations, suggesting that population structure has been substantially reshaped since the Last Glacial Maximum. Our results provide new insights into the Late Pleistocene genetic histories of these three species and demonstrate that direct shotgun sequencing of sediment DNA, without target enrichment methods, can yield genome-wide data informative of ancestry and phylogenetic relationships.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
Depositing User: LivePure Connector
Date Deposited: 24 Oct 2023 01:44
Last Modified: 24 Oct 2023 01:44
DOI: 10.1016/j.cub.2021.06.023


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