Protein film electrochemistry

Butt, Julea N. ORCID:, Jeuken, Lars J. C., Zhang, Huijie, Burton, Joshua A. J. and Sutton-Cook, Alexander L. (2023) Protein film electrochemistry. Nature Reviews Methods Primers, 3. ISSN 2662-8449

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Protein Film Electrochemistry (PFE) is a powerful suite of electroanalytical techniques used to investigate the properties of redox proteins. The proteins under investigation are adsorbed as a (sub-)monolayer film on an electrode surface. Direct electron transfer between the immobilized protein and working electrode gives rise to an electrical current that visualizes and quantifies redox processes occurring within the protein. Advantages of PFE include low sample requirements — typically less than a nanomole protein — high sensitivity and the ability to resolve redox chemistry in the electrochemical potential and time domains. This Primer provides a guide to using PFE for quantitative thermodynamic and kinetic descriptions of half-reactions (redox reactions) and coupled chemical processes, including ligand binding, ligand unbinding and redox catalysis. Applications of PFE in developing biosensors, facilitating energy conversion and resolving enzyme mechanisms are highlighted. Finally, the state-of-the-art and prospects for novel experimental and theoretical approaches are discussed.

Item Type: Article
Additional Information: Acknowledgments: JNB and LJCJ thank Professor Fraser Armstrong for inspiring and guiding their introduction to PFE, and the many students and colleagues with whom they have subsequently collaborated in applying and developing the technique. They are grateful for long-term funding from UK Research Councils, notably BBSRC most recently through grants BB/S000704/1 and BB/S002499/1. JAJB is funded by a PhD studentship from the University of East Anglia and ASC by the UKRI Biotechnology and Biological Sciences Research Council Norwich Research Park Biosciences Doctoral Training Partnership [Grant number BB/T008717/1).
Uncontrolled Keywords: biochemistry, genetics and molecular biology(all) ,/dk/atira/pure/subjectarea/asjc/1300
Faculty \ School: Faculty of Science > School of Chemistry
Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Centre for Photonics and Quantum Science
Faculty of Science > Research Groups > Molecular Microbiology
Faculty of Science > Research Groups > Energy Materials Laboratory
Faculty of Science > Research Groups > Chemistry of Light and Energy
Faculty of Science > Research Groups > Chemistry of Life Processes
Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry
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Depositing User: LivePure Connector
Date Deposited: 01 Sep 2023 09:31
Last Modified: 19 Apr 2024 01:38
DOI: 10.1038/s43586-023-00262-7


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