AePUb promoter length modulates gene expression in Aedes aegypti

Anderson, Michelle A.E., Leftwich, Philip T. ORCID: https://orcid.org/0000-0001-9500-6592, Wilson, Ray, Carabajal Paladino, Leonela Z., Basu, Sanjay, Rooney, Sara, Adelman, Zach N. and Alphey, Luke (2023) AePUb promoter length modulates gene expression in Aedes aegypti. Scientific Reports, 13. ISSN 2045-2322

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Abstract

Molecular tools for modulating transgene expression in Aedes aegypti are few. Here we demonstrate that adjustments to the AePUb promoter length can alter expression levels of two reporter proteins in Ae. aegypti cell culture and in mosquitoes. This provides a simple means for increasing or decreasing expression of a gene of interest and easy translation from cells to whole insects.

Item Type:	Article
Additional Information:	Funding Information: MAEA was funded by Defense Advanced Research Projects Agency (DARPA) award [N66001-17-2-4054] to Kevin Esvelt at MIT. PTL, RW and LZCP were funded by a Wellcome Trust Investigator Award [110117/Z/15/Z] to LA. LA was supported through strategic funding from the UK Biotechnology and Biological Sciences Research Council (BBSRC) to The Pirbright Institute (BBS/E/I/00007033, BBS/E/I/00007038 and BBS/E/I/00007039). The views, opinions and/or findings expressed are those of the authors and should not be interpreted as representing the official views or policies of the U.S. Government. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Uncontrolled Keywords:	general ,/dk/atira/pure/subjectarea/asjc/1000
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	15 Aug 2023 08:30
Last Modified:	27 Jan 2024 03:17
URI:	https://ueaeprints.uea.ac.uk/id/eprint/92862
DOI:	10.1101/2023.08.11.553001

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