Unravelling phenolic metabotypes in the frame of the COMBAT study, a randomized, controlled trial with cranberry supplementation

Tosi, Nicole, Favari, Claudia, Bresciani, Letizia, Flanagan, Emma, Hornberger, Michael ORCID: https://orcid.org/0000-0002-2214-3788, Narbad, Arjan, Del Rio, Daniele, Vauzour, David ORCID: https://orcid.org/0000-0001-5952-8756 and Mena, Pedro (2023) Unravelling phenolic metabotypes in the frame of the COMBAT study, a randomized, controlled trial with cranberry supplementation. Food Research International, 172. ISSN 0963-9969

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Cranberry (poly)phenols may have potential health benefits. Circulating (poly)phenol metabolites can act as mediators of these effects, but they are subjected to an extensive inter-individual variability. This study aimed to quantify both plasma and urine (poly)phenol metabolites following a 12-week intake of a cranberry powder in healthy older adults, and to investigate inter-individual differences by considering the existence of urinary metabotypes related to dietary (poly)phenols. Up to 13 and 67 metabolites were quantified in plasma and urine respectively. Cranberry consumption led to changes in plasma metabolites, mainly hydroxycinnamates and hippuric acid. Individual variability in urinary metabolites was assessed using different data sets and a combination of statistical models. Three phenolic metabotypes were identified, colonic metabolism being the main driver for subject clustering. Metabotypes were characterized by quali-quantitative differences in the excretion of some metabolites such as phenyl-γ-valerolactones, hydroxycinnamic acids, and phenylpropanoic acids. Metabotypes were further confirmed when applying a model only focused on flavan-3-ol colonic metabolites. 5-(3′,4′-dihydroxyphenyl)-γ-valerolactone derivatives were the most relevant metabolites for metabotyping. Metabotype allocation was well preserved after 12-week intervention. This metabotyping approach for cranberry metabolites represents an innovative step to handle the complexity of (poly)phenol metabolism in free-living conditions, deciphering the existence of metabotypes derived from the simultaneous consumption of different classes of (poly)phenols. These results will help contribute to studying the health effects of cranberries and other (poly)phenol-rich foods, mainly considering gut microbiota-driven individual differences.

Item Type: Article
Additional Information: Funding information: This research was partially supported by a Cranberry Institute grant (R204719). The Cranberry Institute was not involved in the design, implementation, analysis, and interpretation of the data. This study also received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (PREDICT-CARE project, grant agreement No 950050).
Uncontrolled Keywords: cranberry,phenolic metabolites,inter-individual variability,metabolic phenotypes,phenyl-γ-valerolactones
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging
Faculty of Medicine and Health Sciences > Research Groups > Mental Health
Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine
Depositing User: LivePure Connector
Date Deposited: 30 Jun 2023 09:30
Last Modified: 06 Jul 2023 09:31
URI: https://ueaeprints.uea.ac.uk/id/eprint/92547
DOI: 10.1016/j.foodres.2023.113187

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