Sensitive dipstick assays for lectin detection, based on glycan-BSA conjugate immobilisation on gold nanoparticles

Hernando, Pedro J., Ivanova, Irina M., Chessa, Simona, Marin, Maria J. ORCID:, Dedola, Simone and Field, Robert A. (2023) Sensitive dipstick assays for lectin detection, based on glycan-BSA conjugate immobilisation on gold nanoparticles. Organic Chemistry Frontiers, 10 (15). pp. 3819-3829. ISSN 2052-4129

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There is an unmet need for simple, reliable, and portable devices for the early detection of pathogens and toxins. In this research work, we explored the development of a rapid carbohydrate-based assay for the detection of glycan-binding proteins, lectins, in relation to the detection of the Ricinus communis phytotoxin ricin due to its potential as a bioterrorism agent. To allow a visual read-out of the test, gold nanoparticles were applied on a paper-based diagnostic device in the format of a dipstick assay. A small collection of carbohydrates was modified with an azidopropyl tether, allowing the functionalisation of alkyne-modified bovine serum albumin through Cu(i)-catalysed alkyne-azide cycloaddition. To further understand the impact of the nanoparticle size and the presentation of glycans on the assay, ca. 150 nm gold nanoshells and ca. 40 nm gold nanoparticles covalently functionalised with glycans were investigated for their performance in the dipstick assays. The detection of a selection of lectins, including R. communis agglutinin 120, a surrogate for ricin, was carried out. The results presented in this work highlight the potential of glyconanoparticles for the development of cost-effective detection tools for pathogens and toxins bearing surface carbohydrate-binding lectins.

Item Type: Article
Additional Information: Acknowledgements: This work was supported by the Marie Skłodowska-Curie Actions (MSCA), as part of the Horizon 2020 programme funded by the EU Commission (Grant Agreement 814102 – Sweet Crosstalk). The authors acknowledge the University of East Anglia and the Quadram Institute, Norwich as hosts for this project.
Uncontrolled Keywords: organic chemistry ,/dk/atira/pure/subjectarea/asjc/1600/1605
Faculty \ School: Faculty of Science > School of Chemistry
UEA Research Groups: Faculty of Science > Research Groups > Centre for Photonics and Quantum Science
Faculty of Science > Research Groups > Chemistry of Life Processes
Faculty of Science > Research Groups > Chemistry of Light and Energy
Faculty of Science > Research Groups > Chemistry of Materials and Catalysis
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Depositing User: LivePure Connector
Date Deposited: 22 Jun 2023 09:31
Last Modified: 18 Aug 2023 13:32
DOI: 10.1039/D3QO00424D

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