Rational design of covalent multiheme cytochrome-graphitic carbon dot biohybrids for photo-induced electron transfer

Zhang, Huijie, Casadevall, Carla, van Wonderen, Jessica, Su, Lin, Butt, Julea ORCID: https://orcid.org/0000-0002-9624-5226, Reisner, Erwin and Jeuken, Lars (2023) Rational design of covalent multiheme cytochrome-graphitic carbon dot biohybrids for photo-induced electron transfer. Advanced Functional Materials. ISSN 1616-301X (In Press)

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Biohybrid systems can combine inorganic light-harvesting materials and whole-cell biocatalysts to exploit solar energy for the production of chemicals and fuels. Whole-cell biocatalysts have an intrinsic self-repair ability and are able to produce a wide variety of multicarbon chemicals in a sustainable way. Current whole-cell biohybrid systems have a yet undefined electron transfer pathway between the light-absorber and metabolic enzymes, limiting rational design. To enable engineering of efficient electron transfer pathways, we developed covalent biohybrids consisting of graphitic nitrogen doped carbon dots (g-N-CDs) and the outer-membrane decaheme protein, MtrC from Shewanella oneidensis MR-1. MtrC is a subunit of MtrCAB protein complex, which provides a direct conduit for bidirectional electron exchange across the Shewanella oneidensis MR-1 outer membrane. The g-N-CDs are functionalized with a maleimide moiety by either carbodiimide chemistry or acyl chloride activation and coupled to a surface-exposed cysteine of a Y657C MtrC mutant. MtrC~g-N-CD biohybrids are characterized by native and denaturing gel electrophoresis, chromatography, microscopy and fluorescence lifetime spectroscopy. In the presence of a sacrificial electron donor, visible light irradiation of the MtrC~g-N-CD biohybrids results in reduced MtrC. The biohybrids might find application in photo-induced transmembrane electron transfer in Shewanella oneidensis MR-1 for chemical synthesis in the future.

Item Type: Article
Faculty \ School: Faculty of Science > School of Chemistry
Faculty of Science > School of Biological Sciences
Depositing User: LivePure Connector
Date Deposited: 25 May 2023 10:32
Last Modified: 26 May 2023 15:31
URI: https://ueaeprints.uea.ac.uk/id/eprint/92186

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