Ginsenosides enhance P2X7-dependent cytokine secretion from LPS-primed rodent macrophages

Dhuna, Kshitija, Helliwell, Ray, de Luca, Simone N., Spencer, Sarah J. and Stokes, Leanne ORCID: (2023) Ginsenosides enhance P2X7-dependent cytokine secretion from LPS-primed rodent macrophages. Purinergic Signalling. ISSN 1573-9538

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The activation of P2X7 is a well-known stimulus for the NLRP3-caspase 1 inflammasome and subsequent rapid IL-1β secretion from monocytes and macrophages. Here we show that positive allosteric modulators of P2X7, ginsenosides, can enhance the release of three important cytokines, IL-1β, IL-6 and TNF-α from LPS-primed rodent macrophages using the J774 mouse macrophage cell line and primary rat peritoneal macrophages. We compared the immediate P2X7 responses in un-primed and LPS-primed macrophages and found no difference in calcium response amplitude or kinetics. These results suggest that under inflammatory conditions positive allosteric modulators are capable of increasing cytokine secretion at lower concentrations of ATP, thus boosting the initial pro-inflammatory signal. This may be important in the control of intracellular infections.

Item Type: Article
Additional Information: Funding information: Supported by an RMIT University PhD Scholarship (KD).
Faculty \ School: Faculty of Science > School of Pharmacy
Depositing User: LivePure Connector
Date Deposited: 05 May 2023 08:30
Last Modified: 05 May 2023 08:30
DOI: 10.1007/s11302-023-09935-0

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