Sulforaphane inhibits foam cell formation and atherosclerosis via mechanisms involving the modulation of macrophage cholesterol transport and the related phenotype

Liu, Shiyan, Zhang, Yuan, Zheng, Xiangyu, Wang, Ziling, Wang, Pan, Zhang, Mengdi, Shen, Mengfan, Bao, Yongping ORCID: https://orcid.org/0000-0002-6425-0370 and Li, Dan (2023) Sulforaphane inhibits foam cell formation and atherosclerosis via mechanisms involving the modulation of macrophage cholesterol transport and the related phenotype. Nutrients, 15 (9). ISSN 2072-6643

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Abstract

Sulforaphane (SFN), an isothiocyanate, is one of the major dietary phytochemicals found in cruciferous vegetables. Many studies suggest that SFN can protect against cancer and cardiometabolic diseases. Despite the proposed systemic and local vascular protective mechanisms, SFN’s potential to inhibit atherogenesis by targeting macrophages remains unknown. In this study, in high fat diet fed ApoE-deficient (ApoE −/−) mice, oral SFN treatment improved dyslipidemia and inhibited atherosclerotic plaque formation and the unstable phenotype, as demonstrated by reductions in the lesion areas in both the aortic sinus and whole aorta, percentages of necrotic cores, vascular macrophage infiltration and reactive oxygen species (ROS) generation. In THP-1-derived macrophages, preadministration SFN alleviated oxidized low-density lipoprotein (ox-LDL)-induced lipid accumulation, oxidative stress and mitochondrial injury. Moreover, a functional study revealed that peritoneal macrophages isolated from SFN-treated mice exhibited attenuated cholesterol influx and enhanced apolipoprotein A-I (apoA-I)- and high-density lipoprotein (HDL)-mediated cholesterol efflux. Mechanistic analysis revealed that SFN supplementation induced both intralesional and intraperitoneal macrophage phenotypic switching toward high expression of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1) and ATP-binding cassette subfamily A/G member 1 (ABCA1/G1) and low expression of peroxisome proliferator-activated receptor γ (PPARγ) and cluster of differentiation 36 (CD36), which was further validated by the aortic protein expression. These results suggest that the regulation of macrophages’ cholesterol transport and accumulation may be mainly responsible for SFN’s potential atheroprotective properties, and the regulatory mechanisms might involve upregulating ABCA1/G1 and downregulating CD36 via the modulation of PPARγ and Nrf2.

Item Type: Article
Additional Information: Funding Information: This research was funded by the National Natural Science Foundation of China (No. 81573143 and 81872612).
Uncontrolled Keywords: apoe-deficient mice,atherosclerosis,cardiovascular disease,cholesterol,cruciferous vegetable,foam cell,isothiocyanate,macrophage,nrf2,sulforaphane,food science,nutrition and dietetics,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/1100/1106
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Groups > Cancer Studies
Faculty of Medicine and Health Sciences > Research Centres > Lifespan Health
Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
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Depositing User: LivePure Connector
Date Deposited: 28 Apr 2023 09:30
Last Modified: 13 Nov 2023 17:55
URI: https://ueaeprints.uea.ac.uk/id/eprint/91899
DOI: 10.3390/nu15092117

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