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Anbu, Sellamuthu 
ORCID: https://orcid.org/0000-0002-7176-330X, Paul, Anup, Surendranath, Kalpana, Sidali, Ahmed and Pombeiro, Armando J. L.
(2021)
Naphthalimide-phenanthroimidazole incorporated new fluorescent sensor for “turn-on” Cu2+ detection in living cancer cells.
Journal of Inorganic Biochemistry, 220.
ISSN 1873-3344
Abstract
In recent years, fluorescent sensors have emerged as attractive imaging probes due to their distinct responses toward bio-relevant metal ions. However, the bioimaging application main barrier is the ‘turn-off’ response toward paramagnetic metal ions such as Cu 2+ under physiological conditions. Herein, we report a new sensor (2-methyl(4-bromo-N-ethylpiperazinyl-1,8-naphthalimido)-4-(1H-phenanthro[9,10-d]imidazole-2-yl) phenol) NPP with multifunctional (Naphthalimide, Piperazine, Phenanthroimidazole) units for fluorescent and colourimetric detection of Cu 2+ in an aqueous medium. Both absorption and fluorescence spectral titration strategies were used to monitor the Cu 2+-sensing property of NPP. The NPP displays a weak emission at ca. 455 nm, which remarkably enhances (⁓3.2-fold) upon selective binding of Cu 2+ over a range of metal ions, including other paramagnetic metal ions (Mn 2+, Fe 3+, Co 2+). The stoichiometry, binding constant (K a) and the LOD (limit of detection) of NPP toward Cu 2+ ions were found to be 1:1, 5.0 (± 0.2) × 10 4 M −1 and 6.5 (± 0.4) × 10 −7 M, respectively. We have also used NPP as a fluorescent probe to detect Cu 2+ in live (human cervical HeLa) cancer cells. The emission intensity of NPP was almost recovered in HeLa cells by incubating ‘in situ’ the derived Cu 2+ complex (NPP-Cu 2+) in the presence of a benchmark chelating agent such as EDTA (ethylenediaminetetraacetate). The fluorescent emission of NPP was reverted significantly in each cycle upon sequencial addition of Cu 2+ and EDTA to the NPP solution. Overall, NPP is a novel, simple, economic and portable sensor that can detect Cu 2+ in biological and environmental scenarios.
| Item Type: | Article |
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| Additional Information: | Funding Information: This work was partially supported by the Fundação para a Ciência e Tecnologia (FCT), Portugal, through project UIDB/00100/2020 of Centro de Química Estrutural. S.A. gratefully acknowledges the FCT for the award of postdoctoral fellowship (Ref.: SFRH/BPD/76451/2011) and the Royal Society of Chemistry (United Kindom) RSC for the Research Fund grant (RF19-7464). A.P. is thankful to the FCT and IST, Portugal, for financial support through “DL/57/2017” (Contracts no. IST-ID/197/2019). Work of the Genome Engineering Laboratory (live cell imaging) is supported by Quintin Hogg Trust “Gene Editors of the Future” and the University of Westminster, London, United Kingdom. This work has also been supported by the RUDN University Strategic Academic Leadership Program. |
| Uncontrolled Keywords: | cu -sensors,mannich base,napthalimide,optical sensors,phenanthroimidazole,turn on sensors,biochemistry,inorganic chemistry,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/1300/1303 |
| Faculty \ School: | Faculty of Science > School of Chemistry |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 20 Mar 2023 14:54 |
| Last Modified: | 30 Jan 2024 03:32 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/91604 |
| DOI: | 10.1016/j.jinorgbio.2021.111466 |
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