Effects of sample heating in FT-Raman spectra of biological materials

Marigheto, N. A., Kemsley, E. K. ORCID: https://orcid.org/0000-0003-0669-3883, Potter, J., Belton, P. S. and Wilson, R. H. (1996) Effects of sample heating in FT-Raman spectra of biological materials. Spectrochimica Acta - Part A Molecular Spectroscopy, 52 (12). pp. 1571-1579.

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The effects of laser-induced sample heating and fluorescence of biopolymers in Fourier transform-Raman spectroscopy were investigated. It was shown that these two phenomena are essentially independent of each other. Methods of quantifying the laser-induced heating were developed and it was shown that measurement of the thermal emission generated by the sample is not sufficient to determine its temperature. The use of a marker compound mixed with the test sample to estimate the sample temperature was explored. Monitoring the temperature-dependent phase transitions of NH4NO3 provided a crude indication of a minimum sample temperature. However, using sulphur as a marker and exploiting the relationship between the intensities of its anti-Stokes and Stokes bands, it was possible to estimate the sample temperature with greater confidence. Using these techniques it was shown that sample temperatures could easily reach approximately 400 K under normal laser power inputs. Such temperatures are likely to be damaging to biological samples and it is concluded that great care must be exercised when using FT-Raman.

Item Type: Article
Faculty \ School: Faculty of Science > School of Environmental Sciences
Faculty of Science > School of Chemistry
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 06 Feb 2023 10:31
Last Modified: 06 Feb 2023 16:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/91003
DOI: 10.1016/0584-8539(96)01732-1

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