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Marigheto, N. A., Kemsley, E. K. 
ORCID: https://orcid.org/0000-0003-0669-3883, Potter, J., Belton, P. S. and Wilson, R. H.
(1996)
Effects of sample heating in FT-Raman spectra of biological materials.
Spectrochimica Acta - Part A Molecular Spectroscopy, 52 (12).
pp. 1571-1579.
Abstract
The effects of laser-induced sample heating and fluorescence of biopolymers in Fourier transform-Raman spectroscopy were investigated. It was shown that these two phenomena are essentially independent of each other. Methods of quantifying the laser-induced heating were developed and it was shown that measurement of the thermal emission generated by the sample is not sufficient to determine its temperature. The use of a marker compound mixed with the test sample to estimate the sample temperature was explored. Monitoring the temperature-dependent phase transitions of NH4NO3 provided a crude indication of a minimum sample temperature. However, using sulphur as a marker and exploiting the relationship between the intensities of its anti-Stokes and Stokes bands, it was possible to estimate the sample temperature with greater confidence. Using these techniques it was shown that sample temperatures could easily reach approximately 400 K under normal laser power inputs. Such temperatures are likely to be damaging to biological samples and it is concluded that great care must be exercised when using FT-Raman.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > School of Environmental Sciences Faculty of Science > School of Chemistry |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 06 Feb 2023 10:31 |
| Last Modified: | 06 Feb 2023 16:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/91003 |
| DOI: | 10.1016/0584-8539(96)01732-1 |
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