Determining miRNA Expression Patterns in Xenopus

Antonaci, Marco, Godden, Alice M. ORCID: and Wheeler, Grant N. ORCID: (2023) Determining miRNA Expression Patterns in Xenopus. In: MicroRNA Detection and Target Identification. Methods in Molecular Biology . Humana Press, pp. 145-154. ISBN 978-1-0716-2981-9

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Whole-mount in situ hybridization (WISH) is a technique that enables temporal and spatial visualization of RNA molecules in an embryo or whole tissue by using a complementary labelled probe. MicroRNAs are short noncoding RNAs of 20–25 nt in length mainly involved in posttranscriptional regulation of gene expression. In this chapter, we describe how to visualize miRNAs in Xenopus laevis or tropicalis by WISH using two different approaches: LNA-WISH to visualize mature miRNAs and pri-miRNA-WISH to visualize the immature form of miRNAs, the pri-miRNAs.

Item Type: Book Section
Additional Information: Funding: MA is funded by the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement no. 860635. AMG was supported by UKRI Biotechnology and Biological Sciences Research Council Norwich Research Park Biosciences Doctoral Training Partnership grants (BB/M011216/1).
Uncontrolled Keywords: development,expression pattern,wish,xenopus,mirna,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/1300/1312
Faculty \ School: Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Wheeler Group
Faculty of Science > Research Groups > Cells and Tissues
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Depositing User: LivePure Connector
Date Deposited: 03 Feb 2023 17:30
Last Modified: 21 Apr 2023 01:49
DOI: 10.1007/978-1-0716-2982-6_11

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