A time-lagged association between the gut microbiome, nestling weight and nestling survival in wild great tits

Davidson, Gabrielle L. ORCID: https://orcid.org/0000-0001-5663-2662, Somers, Shane E., Wiley, Niamh, Johnson, Crystal N., Reichert, Michael S., Ross, R. Paul, Stanton, Catherine and Quinn, John L. (2021) A time-lagged association between the gut microbiome, nestling weight and nestling survival in wild great tits. Journal of Animal Ecology, 90 (4). pp. 989-1003. ISSN 0021-8790

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Natal body mass is a key predictor of viability and fitness in many animals. While variation in body mass and therefore juvenile viability may be explained by genetic and environmental factors, emerging evidence points to the gut microbiota as an important factor influencing host health. The gut microbiota is known to change during development, but it remains unclear whether the microbiome predicts fitness, and if it does, at which developmental stage it affects fitness traits. We collected data on two traits associated with fitness in wild nestling great tits Parus major: weight and survival to fledging. We characterised the gut microbiome using 16S rRNA sequencing from nestling faeces and investigated temporal associations between the gut microbiome and fitness traits across development at Day-8 (D8) and Day-15 (D15) post-hatching. We also explored whether particular microbial taxa were ‘indicator species’ that reflected whether nestlings survived or not. There was no link between mass and microbial diversity on D8 or D15. However, we detected a time-lagged relationship where weight at D15 was negatively associated with the microbial diversity at D8, controlling for weight at D8, therefore reflecting relative weight gain over the intervening period. Indicator species analysis revealed that specificity values were high and fidelity values were low, suggesting that indicator taxa were primarily detected within either the survived or not survived groups, but not always detected in birds that either survived or died. Therefore these indicator taxa may be sufficient, but not necessary for determining either survival or mortality, perhaps owing to functional overlap in microbiota. We highlight that measuring microbiome-fitness relationships at just one time point may be misleading, especially early in life. Instead, microbial-host fitness effects may be best investigated longitudinally to detect critical development windows for key microbiota and host traits associated with neonatal weight. Our findings should inform future hypothesis testing to pinpoint which features of the gut microbial community impact on host fitness, and when during development this occurs. Such confirmatory research will shed light on population level processes and could have the potential to support conservation.

Item Type: Article
Additional Information: Funding Information: This work was supported by Science Foundation Ireland by way of funding to APC Microbiome Ireland, Cork, Ireland. GLD, SES, MRC and JLQ, etc. were supported by funding from the European Research Council under the European Union's Horizon 2020 Programme (FP7/2007‐2013)/ERC Consolidator Grant ‘Evoecocog’ Project No. 617509, awarded to JLQ. GLD is funded by a Leverhulme Trust Early Career Fellowship (ECF‐2018‐700) with matched funding from the Sir Isaac Newton Trust. Publisher Copyright: © 2021 The Authors. Journal of Animal Ecology published by John Wiley & Sons Ltd on behalf of British Ecological Society
Uncontrolled Keywords: fitness,great tit,gut microbiome,microbial diversity,microbiota,survival,viability,weight,ecology, evolution, behavior and systematics,animal science and zoology ,/dk/atira/pure/subjectarea/asjc/1100/1105
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 13 Jan 2023 17:31
Last Modified: 13 Jan 2023 17:31
URI: https://ueaeprints.uea.ac.uk/id/eprint/90575
DOI: 10.1111/1365-2656.13428


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