Absence of p300 induces cellular phenotypic changes characteristic of epithelial to mesenchyme transition

Krubasik, D., Iyer, N. G., English, W. R. ORCID: https://orcid.org/0000-0003-3024-2441, Ahmed, A. A., Vias, M., Roskelley, C., Brenton, J. D., Caldas, C. and Murphy, G. (2006) Absence of p300 induces cellular phenotypic changes characteristic of epithelial to mesenchyme transition. British Journal of Cancer, 94 (9). pp. 1326-1332. ISSN 1532-1827

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p300 is a transcriptional cofactor and prototype histone acetyltransferase involved in regulating multiple cellular processes. We generated p300 deficient (p300−) cells from the colon carcinoma cell line HCT116 by gene targeting. Comparison of epithelial and mesenchymal proteins in p300− with parental HCT116 cells showed that a number of genes involved in cell and extracellular matrix interactions, typical of ‘epithelial to mesenchyme transition’ were differentially regulated at both the RNA and protein level. p300− cells were found to have aggressive ‘cancer’ phenotypes, with loss of cell–cell adhesion, defects in cell–matrix adhesion and increased migration through collagen and matrigel. Although migration was shown to be metalloproteinase mediated, these cells actually showed a downregulation or no change in the level of key metalloproteinases, indicating that changes in cellular adhesion properties can be critical for cellular mobility.

Item Type: Article
Uncontrolled Keywords: sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Depositing User: LivePure Connector
Date Deposited: 15 Dec 2022 10:31
Last Modified: 19 Oct 2023 03:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/90198
DOI: 10.1038/sj.bjc.6603101

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