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Roghi, Christian, Jones, Louise, Gratian, Matthew, English, William R 
ORCID: https://orcid.org/0000-0003-3024-2441 and Murphy, Gillian
(2010)
Golgi reassembly stacking protein 55 interacts with membrane‐type (MT) 1‐matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1‐MMP zymogen.
FEBS Journal, 277 (15).
pp. 3158-3175.
ISSN 1742-464X
Abstract
Membrane-type 1 matrix metalloproteinase (MT1-MMP) is a proteinase involved in the remodelling of extracellular matrix and the cleavage of a number of substrates. MT1-MMP is synthesized as a zymogen that requires intracellular post-translational cleavage to gain biological activity. Furin, a member of the pro-protein convertase family, has been implicated in the proteolytic removal of the MT1-MMP prodomain sequence. In the present study, we demonstrate a role for the peripheral Golgi matrix protein GRASP55 in the furin-dependent activation of MT1-MMP. MT1-MMP and furin were found to co-localize with Golgi reassembly stacking protein 55 (GRASP55). Further analysis revealed that GRASP55 associated with the cytoplasmic domain of both proteases and that the LLY573 motif in the MT1-MMP intracellular domain was crucial for the interaction with GRASP55. Overexpression of GRASP55 was found to enhance the formation of a complex between MT1-MMP and furin. Finally, we report that disruption of the interaction between GRASP55 and furin led to a reduction in pro-MT1-MMP activation. Taken together, these data suggest that GRASP55 may function as an adaptor protein coupling MT1-MMP with furin, thus leading to the activation of the zymogen.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Medicine and Health Sciences > Norwich Medical School |
| Depositing User: | LivePure Connector |
| Date Deposited: | 15 Dec 2022 10:31 |
| Last Modified: | 12 Jan 2023 09:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/90194 |
| DOI: | 10.1111/j.1742-4658.2010.07723.x |
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