Comparison of the genetic basis of biofilm formation between Salmonella Typhimurium and Escherichia coli

Holden, Emma R., Yasir, Muhammad, Turner, A. Keith, Charles, Ian G. and Webber, Mark A. (2022) Comparison of the genetic basis of biofilm formation between Salmonella Typhimurium and Escherichia coli. Microbial Genomics, 8 (11). ISSN 2057-5858

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Abstract

Most bacteria can form biofilms, which typically have a life cycle from cells initially attaching to a surface before aggregation and growth produces biomass and an extracellular matrix before finally cells disperse. To maximize fitness at each stage of this life cycle and given the different events taking place within a biofilm, temporal regulation of gene expression is essential. We recently described the genes required for optimal fitness over time during biofilm formation in Escherichia coli using a massively parallel transposon mutagenesis approach called TraDIS-Xpress. We have now repeated this study in Salmonella enterica serovar Typhimurium to determine the similarities and differences in biofilm formation through time between these species. A core set of pathways involved in biofilm formation in both species included matrix production, nucleotide biosynthesis, flagella assembly and LPS biosynthesis. We also identified several differences between the species, including a divergent impact of the antitoxin TomB on biofilm formation in each species. We observed deletion of tomB to be detrimental throughout the development of the E. coli biofilms but increased biofilm biomass in S. Typhimurium. We also found a more pronounced role for genes involved in respiration, specifically the electron transport chain, on the fitness of mature biofilms in S. Typhimurium than in E. coli and this was linked to matrix production. This work deepens understanding of the core requirements for biofilm formation in the Enterobacteriaceae whilst also identifying some genes with specialised roles in biofilm formation in each species.

Item Type: Article
Additional Information: Funding Information: E.R.H. was supported by a studentship funded by the Quadram Institute Bioscience. The authors gratefully acknowledge the support of the Biotechnology and Biological Sciences Research Council (BBSRC); A.K.T., M.Y., I.G.C. and M.A.W. were supported by the BBSRC Institute Strategic Programme Microbes in the Food Chain BB/R012504/1 and its constituent project BBS/E/F/000PR10349. Publisher Copyright: © 2022 The Authors.
Uncontrolled Keywords: functional genomics,tradis,maop,nuo,tomb,transposon mutagenesis,epidemiology,microbiology,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/2700/2713
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: LivePure Connector
Date Deposited: 15 Dec 2022 04:02
Last Modified: 15 Dec 2022 04:02
URI: https://ueaeprints.uea.ac.uk/id/eprint/90123
DOI: 10.1099/mgen.0.000885

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